| 半套式PCR方法检测猪附红细胞体病的建立 |
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| 引用本文： | 袁聪俐,杨志彪,朱建国,崔立,华修国.半套式PCR方法检测猪附红细胞体病的建立[J].上海交通大学学报(农业科学版),2006,24(1):58-60,74. |
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| 作者姓名： | 袁聪俐 杨志彪 朱建国 崔立 华修国 |
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| 作者单位： | 上海交通大学,农业与生物学院,上海,201101 |
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| 摘 要： | 建立一种客观敏感的附红细胞体病早期实验室诊断方法。本实验采用已公布的附红细胞体序列设计半套式引物进行PCR扩增,对扩增出得特异性条带进行序列分析。镜检结果阳性率为75%,PCR检测结果阳性率为90%,测序结果与已公布的序列同源性达99.9%。半套式PCR方法比镜检高效准确,适合附红细胞体病的早期诊断。
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| 关 键 词： | 半套式PCR 附红细胞体 感染率 |
| 文章编号： | 1671-9964（2006）01-0058-03 |
| 收稿时间： | 2005-11-10 |
| 修稿时间： | 2005-11-10 |
Establishment of Half-nested PCR Method on Eperythrozoon Diagnosis |
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| YUAN Cong-li,YANG Zhi-biao,ZHU Jian-guo,CUI Li,HUA Xiu-guo.Establishment of Half-nested PCR Method on Eperythrozoon Diagnosis[J].Journal of Shanghai Jiaotong University (Agricultural Science),2006,24(1):58-60,74. |
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| Authors: | YUAN Cong-li YANG Zhi-biao ZHU Jian-guo CUI Li HUA Xiu-guo |
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| Institution: | School of Agriculture and Biology ,Shanghai Jiaotong University,Shanghai 201101, China |
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| Abstract: | Establishing an efficient method for eperythrozoonosis diagnosis.In this study,half-nested PCR was performed to detect the samples after microscopic examination.The result of microscopic examination and half-nested PCR showed the infection rate was 75 % and 90 % respectively,which demonstrated that PCR was the best way for early and chronic eperythrozoonosis diagnosis. |
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| Keywords: | half-nested PCR eperythrozoon infection rate |
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