| 番茄耐盐愈伤组织的筛选及显微结构观察 |
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| 引用本文： | 戴伟民,赵艳,等.番茄耐盐愈伤组织的筛选及显微结构观察[J].上海交通大学学报(农业科学版),2001,19(2):112-116. |
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| 作者姓名： | 戴伟民 赵艳 |
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| 作者单位： | 上海交通大学植物科学系 |
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| 基金项目： | 上海市科委资助项目(983913013) |
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| 摘 要： | 以番茄下胚轴为外植体诱导愈伤组织，转入含10g/LNaCl的筛选培养基上培养，经过3代高盐直接筛选，获得耐盐愈伤组织，进行电镜观察，结果表明，存活下来的耐盐绿色愈伤组织细胞及细胞器（与对照相比）形态结构正常，代谢活动旺盛，而处于死亡状态的浅褐色愈伤组织和解体死亡的黑色愈伤组织细胞及细胞器形态结构异常。
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| 关 键 词： | 番茄 耐盐愈伤组织 细胞显微结构 |
| 文章编号： | 1000-193(2001)02-0112-05 |
| 修稿时间： | 2000年12月11 |
Selection of NaCl-tolerant calli and observation of microstructure |
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| DAI Wei min,ZHAO Yan,CAI Run,PAN Jun song,HE Huan le,LU Ya jun.Selection of NaCl-tolerant calli and observation of microstructure[J].Journal of Shanghai Jiaotong University (Agricultural Science),2001,19(2):112-116. |
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| Authors: | DAI Wei min ZHAO Yan CAI Run PAN Jun song HE Huan le LU Ya jun |
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| Abstract: | The calli produced from hypocotyl of tomato,that had been maintained for 60d, were transferred to media supplemented with NaCl 10g/L for callus selection.NaCl tolerant calli were obtained by three generations of selections.On medium containing NaCl 10g/L,cells and their organelleles in NaCl tolerant calli had normal morphology and structure,and vigorous metabolism;but in untolerant calli,the majority of cells turned towards dying. |
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| Keywords: | Tomato NaCl tolerant calli Microstructure of cell |
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