| 中华蜜蜂MRJP7基因的原核表达及多克隆抗体的制备 |
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| 引用本文： | 李艳,陈艳霞,沈立荣,张传溪.中华蜜蜂MRJP7基因的原核表达及多克隆抗体的制备[J].上海交通大学学报(农业科学版),2008,26(2):109-113. |
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| 作者姓名： | 李艳 陈艳霞 沈立荣 张传溪 |
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| 作者单位： | 浙江大学,昆虫科学研究所,杭州310029 |
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| 摘 要： | 用PCR方法从含中华蜜蜂Accmrjp7基因的质粒DNA模板中扩增出MRJP7成熟肽编码区片段，将该片段克隆入原核表达载体pGEX-4T-2，在大肠杆菌（Escherichia coli）BL21（DE3）中进行融合表达，SDS—PAGE和薄层扫描显示，该基因得到高效表达，表达产物大小约为66kDa，占细胞总蛋白的22．8％。利用割胶回收的方法，获得目的蛋白，注射兔子制备多克隆抗体，并利用所获抗体进行Western blot印迹分析，检测MRJPs，获得特异性条带。
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| 关 键 词： | 中华蜜蜂 蜂王浆 AccMRJP7 原核表达 多克隆抗体 |
| 文章编号： | 1671-9964(2008)02-0109-05 |
| 修稿时间： | 2007年3月20日 |
Prokaryotic Expression of mrjp7 of Apis cerana cerana and Preparation of Its Polyclonal Antibody |
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| LI Yan CHEN Yan-xia SHEN Li-rong ZHA NG Chuan-xi.Prokaryotic Expression of mrjp7 of Apis cerana cerana and Preparation of Its Polyclonal Antibody[J].Journal of Shanghai Jiaotong University (Agricultural Science),2008,26(2):109-113. |
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| Authors: | LI Yan CHEN Yan-xia SHEN Li-rong ZHA NG Chuan-xi |
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| Institution: | LI Yan CHEN Yan-xia SHEN Li-rong ZHA NG Chuan-xi (Institute of Insect Sciences,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | The coding region of MRJP7 mature peptide was amplified by PCR from Apis cerana cerana cDNA library and cloned into the prokaryotic expression vector pGEX-4T-2 for fusion expression in E.coli.The SDS-PAGE and thin-layer scanning results showed that the expressed GST-MRJF7 fusion protein was about 66 kDa in size and about 22.8% in proportion to the total bacterial proteins.The expressed products were retrieved from the SDS-PAGE gels and used to immunize the rabbit to produce the polyclonal antibody against M... |
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| Keywords: | Apis cerana cerana royal jelly AccMRJP7 prokaryotic expression polyclonal antibody |
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