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猪圆环病毒2型、猪繁殖与呼吸综合征病毒及猪细小病毒混合感染的流行病学调查
引用本文:崔立,姚从斌,芦银华,陈溥言,华修国.猪圆环病毒2型、猪繁殖与呼吸综合征病毒及猪细小病毒混合感染的流行病学调查[J].上海交通大学学报(农业科学版),2006,24(4):330-334.
作者姓名:崔立  姚从斌  芦银华  陈溥言  华修国
作者单位:1. 上海交通大学,农业与生物学院,上海 201101
2. 南京农业大学,动物医学院,南京 210095
基金项目:上海市科技兴农科技攻关项目
摘    要:根据Genbank中PRRSV ORF7及PPVVP2基因序列设计合成引物,建立了分别用于检测PRRSV、PPV的RT-PCR及PCR方法。应用建立的方法对38个PCV2阳性病料进行了PRRSV及PPV检测,以确定猪群中PCV2与PRRSV和/或PPV混合感染情况,结果表明,15份样品表现为PRRSV与PCV2混合感染,占样品总数的39.4%;2份样品表现为PCV2与PPV混合感染,占5.3%。另外,还有一定比例的三重感染,共3个样品,占7.9%。由此可见,猪群中PCV2与PRRSV及PPV混合感染比较普遍。

关 键 词:猪圆环病毒2型  猪繁殖与呼吸综合征病毒  猪细小病毒  混合感染
文章编号:1671-9964(2006)04-0330-05
收稿时间:2006-03-07
修稿时间:2006年3月7日

Epidemiology Investigation of Co-infection with Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Parvovirus (PPV)
CUI Li,YAO Gong-bin,LU Yin-hu,CHEN Pu-yan,HUA Xiu-guo.Epidemiology Investigation of Co-infection with Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Parvovirus (PPV)[J].Journal of Shanghai Jiaotong University (Agricultural Science),2006,24(4):330-334.
Authors:CUI Li  YAO Gong-bin  LU Yin-hu  CHEN Pu-yan  HUA Xiu-guo
Institution:1. School of Agriculture and Biology, Shanghai Jiaotong Univ., Shanghai 201101; 2. School of Animal Medicine, Nanjing Agriculture Univ., Nanjing 210095, China
Abstract:To establish a RT-PCR and a PCR diagnosis method for PRRSV and PPV respectively, two pairs of primers were designed according to the published PRRSV ORF7 gene and PPV VP2 gene. Thirty eight PCV2 positive samples were examined by RT-PCR or PCR method to confirm the mixed infection situation of PCV2, PRRSV, and PPV. However, fifteen samples were examined mixed infection with PRRSV and PCV2, accounting for 39.4% for the total volume, while two sampled were with PCV2 and PPV, accounting for 5.3%, accordingly. Moreover, there were three samples of ternary infection by PCV2, PRRSV, and PPV, which accounted for 7.9% for the total volume. The result indicated the prevalent mixed infection situation of PCV2, PRRSV, and PPV in the herd.
Keywords:porcine circovirus type 2 (PCV2)  porcine reproductive and respiratory byndrome virus (PRRSV)  porcine parvovirus (PPV)  co-infection  
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