| 花生矮化病毒3种PCR检测方法的建立和比较 |
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| 引用本文： | 代欢欢,陈舜胜,杨翠云,于翠.花生矮化病毒3种PCR检测方法的建立和比较[J].上海交通大学学报(农业科学版),2011,29(3):57-1. |
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| 作者姓名： | 代欢欢 陈舜胜 杨翠云 于翠 |
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| 作者单位： | 1. 上海海洋大学食品学院,上海,201300
2. 上海出入境检验检疫局,上海,200135 |
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| 基金项目： | 上海出入境检验检疫局科技项目(HK10-10) |
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| 摘 要： | 建立了花生矮化病毒（PSV）普通RT-PCR、SYBR Green I荧光RT-PCR和免疫捕获RT-PCR检测方法,并比较了它们的检测灵敏度。结果表明,以阳性对照为样品,普通RT-PCR灵敏度达到10-4,SYBR Green I荧光RT-PCR和免疫捕获RT-PCR的检测灵敏度相当,相对灵敏度均可达到10-6;检测混有PSV的大豆叶片样品,普通RT-PCR灵敏度为10-1,SYBR Green I荧光RT-PCR和免疫捕获RT-PCR的检测灵敏度为10-5;SYBR Green I荧光RT-PCR和免疫捕获RT-PCR方法比直接RT-PCR灵敏度高至少100倍,尤其在带毒植物叶片检测中优势更明显,而且灵敏、简便、同时重复性好。
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| 关 键 词： | 花生矮化病毒 PCR 检测方法 灵敏度 |
Development and Comparison of Three PCR Methods for Detecting Peanut stunt virus |
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| DAI Huan-huan,CHEN Shun-sheng,YANG Cui-yun,YU Cui.Development and Comparison of Three PCR Methods for Detecting Peanut stunt virus[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(3):57-1. |
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| Authors: | DAI Huan-huan CHEN Shun-sheng YANG Cui-yun YU Cui |
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| Institution: | DAI Huan-huan1,CHEN Shun-sheng1,YANG Cui-yun2,YU Cui2(1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201300,China,2.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China) |
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| Abstract: | RT-PCR,SYBR Green I real-time RT-PCR and immune-capture RT-PCR approaches were developed for detection of Peanut stunt virus(PSV),and their detecting sensitivity was compared.The results showed that the sensitivity of RT-PCR for detection of PSV was 10-4,whereas the sensitivity of SYBR Green I real-time RT-PCR and immune-capture RT-PCR was 10-6,respectively.The detection limit of RT-PCR for soybean leaf samples contaminated with PSV was 10-1,while that of SYBR Green I realtime RT-PCR and immune-capture RT-P... |
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| Keywords: | Peanut stunt virus PCR detection approach sensitivity |
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