| 用大肠杆菌(Escherichia coli)表达诺如病毒P粒子 |
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| 引用本文： | 宋维,李善爽,杨桂华,赵凌侠.用大肠杆菌(Escherichia coli)表达诺如病毒P粒子[J].上海交通大学学报(农业科学版),2011,29(2):37-42. |
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| 作者姓名： | 宋维 李善爽 杨桂华 赵凌侠 |
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| 作者单位： | 上海交通大学农业与生物学院,植物生物技术研究中心,上海200240 |
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| 基金项目： | 国家自然科学基金项目(31071810); 国家高技术研究发展计划(863计划)项目(2007AA100503) |
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| 摘 要： | 为了研制番茄口服疫苗,按番茄偏爱密码子设计合成了编码诺如病毒(Norovirus,NVs)VA387毒株P粒子(P particles)基因PGENE(1039 bp),并在该基因5′端添加了编码His-tag(6×His)的DNA序列.将构建的原核表达载体pMAL-c2-PGENE导入大肠杆菌(Escherichia...
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| 关 键 词： | 诺如病毒 P粒子 原核表达系统 亚基疫苗 |
| 收稿时间： | 2011/1/28 0:00:00 |
Express Recombinant Norovirus P particles in Escherichia coli |
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| SONG Wei,LI Sang-shuang,YANG Gui-hua and ZHAO Ling-xia.Express Recombinant Norovirus P particles in Escherichia coli[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(2):37-42. |
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| Authors: | SONG Wei LI Sang-shuang YANG Gui-hua and ZHAO Ling-xia |
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| Institution: | SONG Wei,LI Sang-shuang,YANG Gui-hua,ZHAO Ling-xia(Plant Biotechnology Research Center,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | In order to develop edible vaccines via tomato expression system,the gene coding the VA387 Norovirus P particle（designate PGENE）（1 039 bp）was designed and synthesized according to tomato codon usage bias,and DNA sequence of the coded 6×His-tag was fused into the upstream of the PGENE for purification.Prokaryotic expression vector of the pMAL-c2-PGENE was constructed,and introduced into BL21（Escherichia coli）.The recombinant P particle protein was successfully expressed in BL21（E.coli）by 1 mmol/L IPTG（Isopropyl β-D-1-thiogalactopyranoside） induction.Based on the results of SDS-PAGE assay,the optimized induction conditions including temperature（37 ℃） and time（6 h） was established.The identity of P particle and immunocompetence were confirmed by Western blot.This research will provide indispensable preparation to develop eatable subunit vaccines through the tomato fruit expression systems. |
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| Keywords: | noroviruse P particles prokaryotic expression system subunit vaccine |
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