水葫芦生防真菌Cercosporasp.FJ24的分离、鉴定及其分子生物学研究

从福建省得到被致病菌强烈感染的水葫芦植株,从中分离到致病菌菌株FJ24。形态学鉴定表明,该致病菌属于尾孢属。对该菌株的ITS区、EF-1、β-Tub、His-H3基因进行PCR扩增,测序结果显示其与目前已经分离、发现的多种尾孢属水葫芦致病菌存在着99%以上的序列相似性,可以认为其属于半知菌类（无性类）、丝孢纲、丝孢目、淡丛梗孢科、尾孢属的Cercospora piaropi。

Isolation,Identification and Molecular Biological Studies on the Pathogenic Strain Cercospora sp. FJ24 in Water Hyacinth

Water hyacinth severely infected by pathogenic fungi was obtained from Fujian Province of China and a bacterial strain named FJ24 with high pathogenicity was isolated.Morphological identification showed that this pathogenic fungi strain belonged to Cercospora.The results from the sequence determination of the PCR products of ITS,EF-1,β-Tub and His-H3 indicated that there was affinity of above 99% between FJ24 and other pathogenic strains of Cercospora,suggesting that FJ24 strain belonged to the Cercospora piaropi of Imperfect fungi,Hyphomycetes,Moniliales,Moniliaceae.