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长春花叶片三种生物碱含量及合成基因表达的初步研究
引用本文:田跃胜,陆平,王名雪,唐克轩,赵静雅.长春花叶片三种生物碱含量及合成基因表达的初步研究[J].上海交通大学学报(农业科学版),2011,29(2):6-10.
作者姓名:田跃胜  陆平  王名雪  唐克轩  赵静雅
作者单位:1. 上海交通大学农业与生物学院,上海,200240
2. 华南理工大学生物科学与工程学院,广州,510006
基金项目:国家高技术研究发展计划(863计划)项目(2007AA10Z189)
摘    要:为探明长春花叶片中生物碱的动态变化和生物碱合成途径中的关键酶基因的表达量,利用高效液相色谱技术(HPLC)测定了5对真叶期、开花前期、出现花蕾期、出现开花期、成熟期1和成熟期2等6个生长阶段的长春花幼嫩、中等成熟、成熟三种叶片的生物碱(文多灵、长春质碱、长春碱)的含量.幼嫩叶片中文多灵、长春质碱和成熟叶片中长春碱的含量...

关 键 词:长春花  萜类吲哚生物碱  荧光定量PCR  高压液相色谱
收稿时间:2011/2/21 0:00:00

Preliminary Studies on Dynamic Change of Three Alkaloids and Expression of The Associated Biosynthetic Genes In Catharathus Roseus Leaves
TIAN Yue-sheng,LU Ping,WANG Ming-xue,TANG Ke-xua and ZHAO Jing-ya.Preliminary Studies on Dynamic Change of Three Alkaloids and Expression of The Associated Biosynthetic Genes In Catharathus Roseus Leaves[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(2):6-10.
Authors:TIAN Yue-sheng  LU Ping  WANG Ming-xue  TANG Ke-xua and ZHAO Jing-ya
Institution:TIAN Yue-sheng1,LU Ping2,WANG Ming-xue1,TANG Ke-xuan1,ZHAO Jing-ya1(1.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,2.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 51006,China)
Abstract:In order to clarify the dynamic change of three alkaloids(TIAs including vindoline,catharanthine and vinblastine) and the expression of the associated biosynthetic genes in the Catharathus Roseus leaves(young,medium and old) at different growing stages,high performance liquid chromatography was applied to determine the contents of TIAs at the leaf period,pre-floweringperiod,bud stage,flowering period,fully-mature period 1 and fully-mature period 2 in five pairs of leaves from Catharathus Roseus.In addition,...
Keywords:Catharanthus Roseus L  G  don  Terpenoid indole alkaloids(TIAs)  fluroscent real-time quantitative PCR(FQ-PCR)  high-performance liquid chromatography(HPLC)  
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