| 农杆菌介导棉花茎尖遗传转化体系优化 |
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| 引用本文： | 刘明月,左开井.农杆菌介导棉花茎尖遗传转化体系优化[J].上海交通大学学报(农业科学版),2011,29(2):25-31. |
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| 作者姓名： | 刘明月 左开井 |
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| 作者单位： | 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2006AA10Z180); 国家转基因重大专项(2009ZX08009-091B) |
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| 摘 要： | 生根困难和转化效率较低是农杆菌介导棉花茎尖遗传转化体系的应用瓶颈。本研究以柯字棉312为研究材料,探讨茎尖遗传转化中植物生长调节剂、生根诱导时间、无菌苗型、针刺处理等对茎尖生根率、茎尖形态以及抗性苗产生率等的影响。结果表明,1.5 mg/L NAA能够有效提高生根率,促进茎尖伸长;生根诱导时间是生根率的重要影响因子;经多次针刺处理的正常苗抗性苗率较高;培养5 d的正常苗茎尖生长速度和状态优于黄化苗。
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| 关 键 词： | 棉花 农杆菌介导 茎尖 |
| 收稿时间： | 2011/1/22 0:00:00 |
Optimization of Agrobacterium-mediated Transformation System of Shoot Apex |
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| LIU Ming-yue and ZUO Kai-jing.Optimization of Agrobacterium-mediated Transformation System of Shoot Apex[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(2):25-31. |
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| Authors: | LIU Ming-yue and ZUO Kai-jing |
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| Institution: | LIU Ming-yue,ZUO Kai-jing(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Low root-inducing rate and transformation efficiency are the tough problems affecting Agrobacterium-mediated genetic transformation of cotton shoot apex system.Using shoot apex of Coker 312,this paper investigated the effect of plant growth regulators,root-inducing time,different kind of sterile seedlings and acupuncture on the root-inducing rate,transformation on efficiency and morphology.The result showed that 1.5 mg/L NAA could not only promote root-inducing rate but also accelerate its growing speed;Foo... |
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| Keywords: | cotton Agrobacterium-mediated shoot apex |
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