| 基于受体捕获的人源诺如病毒检测方法的优化及应用 |
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| 引用本文： | 周震寰,刘丹蕾,张麟,崔妍,史贤明,王大鹏.基于受体捕获的人源诺如病毒检测方法的优化及应用[J].上海交通大学学报(农业科学版),2017(5):83-88. |
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| 作者姓名： | 周震寰 刘丹蕾 张麟 崔妍 史贤明 王大鹏 |
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| 作者单位： | 上海交通大学 农业与生物学院,中美食品安全联合研究中心,上海200240,上海交通大学 农业与生物学院,中美食品安全联合研究中心,上海200240,上海食品药品监督管理局,上海200003,上海交通大学 农业与生物学院,中美食品安全联合研究中心,上海200240,上海交通大学 农业与生物学院,中美食品安全联合研究中心,上海200240,上海交通大学 农业与生物学院,中美食品安全联合研究中心,上海200240 |
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| 基金项目： | 国家高技术研究发展计划(2012AA101601);国家自然科学基金(31301475) |
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| 摘 要： |
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| 关 键 词： | 人源诺如病毒 受体捕获 原位捕获反转录实时定量PCR 贝类 |
| 收稿时间： | 2016/11/27 0:00:00 |
Optimization and Application of Receptors Capturing Based Human Noroviruses Assay |
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| ZHOU Zhen-huan,LIU Dan-lei,ZHANG Lin,CUI Yan,SHI Xian-ming and WANG Da-peng.Optimization and Application of Receptors Capturing Based Human Noroviruses Assay[J].Journal of Shanghai Jiaotong University (Agricultural Science),2017(5):83-88. |
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| Authors: | ZHOU Zhen-huan LIU Dan-lei ZHANG Lin CUI Yan SHI Xian-ming and WANG Da-peng |
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| Institution: | MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 200240, China,MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 200240, China,Shanghai Municipal Food and Drug Administration, Shanghai 200003, China,MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 200240, China,MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 200240, China and MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 200240, China |
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| Abstract: | Based on the establishment of in situ capture real-time quantitative RT-PCR (ISC-RT-qPCR),this study aimed at optimizing this new detection method to detect infectious HuNoVs,and applying on detections of HuNoVs in shellfish from retail markets.Stool samples,3010 (GI) and 3009 (GII),were used to optimize the conditions of ISC-RT-qPCR.The detection conditions of ISC-RT-qPCR,including coating concentration of porcine gastric mucin (PGM),incubation time and incubation pH,were optimized.Forty shellfish samples (clam,astarte,razor clam,scallop,and abalone) were randomly collected from retail markets to detect infectious HuNoVs particles.The results indicated that the optimum conditions were:1.0 mg/mL for PGM coating concentration,30 min for incubation time,and incubation pH 3.0 for GI and pH 7.0 for GII.In all 40 samples,the total and double positive rates of HuNoVs were 22.5% and 5.0%,respectively;detection rates of GI and GII HuNoVs were 17.5% and 10.0%,respectively.In all 5 kinds of shellfish,clam has the highest detection rates of both GI and GII HuNoVs.ISC-RT-qPCR is a good candidate method on detecting infectious HuNoVs in retail shellfish samples,thus provide another choice for detection and monitoring of HuNoVs in food samples. |
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| Keywords: | human noroviruses receptors capturing in situ capture RT-qPCR shellfish |
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