| 青蒿非分泌型腺毛特异TPS7基因启动子的克隆及功能分析 |
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| 引用本文： | 陈俏,付雪晴,颜廷祥,石璞,郝小龙,唐克轩.青蒿非分泌型腺毛特异TPS7基因启动子的克隆及功能分析[J].上海交通大学学报(农业科学版),2016(6):21-28. |
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| 作者姓名： | 陈俏 付雪晴 颜廷祥 石璞 郝小龙 唐克轩 |
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| 作者单位： | 上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240,上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240,上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240,上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240,上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240,上海交通大学 农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240 |
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| 基金项目： | 国家836计划(2011AA100605) |
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| 摘 要： | 研究运用基因组步移法克隆了长度为1 167bp的青蒿启动子proTPS7,经生物信息学分析发现了proTPS7中的多个顺式调控元件。构建启动子与GUS融合载体,稳定转化青蒿并对转基因植株进行GUS染色实验,结果表明该启动子能驱动报告基因在非分泌型腺毛中的特异表达。对转基因青蒿喷洒甲基茉莉酸和脱落酸,3h后GUS基因表达水平有所上调,说明proTPS7能受到上述2种激素的诱导。
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| 关 键 词： | 青蒿 萜类合成酶 非分泌型腺毛 GUS染色 顺式调控元件 |
| 收稿时间： | 2016/1/6 0:00:00 |
Cloning and Analysis of the Promoter of a Non-Glandular Trichome Specific Gene TPS7 in Artemisia annua L. |
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| CHEN Qiao,FU Xue-qing,YAN Ting-xiang,SHI Pu,HAO Xiao-long and TANG Ke-xuan.Cloning and Analysis of the Promoter of a Non-Glandular Trichome Specific Gene TPS7 in Artemisia annua L.[J].Journal of Shanghai Jiaotong University (Agricultural Science),2016(6):21-28. |
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| Authors: | CHEN Qiao FU Xue-qing YAN Ting-xiang SHI Pu HAO Xiao-long and TANG Ke-xuan |
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| Institution: | SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China and SJTU-Fudan-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | A promoter of 1 167 bp called proTPS7 was isolated from Artemisia annua L.by genomic walking.Several cis-acting elements were discovered via bioinformatics.Then it was fused to GUS reporter gene and transformed into A.annua.GUS staining assay showed that the promoter initiated non-glandular trichome specific expression of GUS gene.GUS expression level in transgenic A.annua was increased 3 hours after MeJA and ABA treatments,indicating that proTPS7 was hormone-responsive. |
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| Keywords: | Artemisia annua L terpene synthase non-glandular trichome GUS staining cis-acting regulatory element |
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