| 视黄酸及干扰素对胃癌细胞系MKN45中p16,p21,c-myc基因表达的影响 |
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| 引用本文： | 胡劲辉,刘尧,孙鹏,朱聪,韦庆文.视黄酸及干扰素对胃癌细胞系MKN45中p16,p21,c-myc基因表达的影响[J].吉林林学院学报,2010(3):229-232. |
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| 作者姓名： | 胡劲辉 刘尧 孙鹏 朱聪 韦庆文 |
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| 作者单位： | [1]深圳市龙岗中心医院,广东深圳518116 [2]深圳市药品监督所,广东深圳518114 [3]深圳市南山区人民医院,广东深圳518052 [4]深圳市平湖卫生监督所,广东深圳518111 |
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| 基金项目： | 深圳市龙岗区科技计划项目（YW2007064） |
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| 摘 要： | 目的探讨全反式视黄酸及干扰素两种因子对胃癌MKN45细胞的影响.方法将视黄酸及干扰素同时加入胃癌细胞系MKN45中进行细胞培养,用MTT法测定细胞的生长状况,并通过Northern blot和免疫组化测定p16,p21及c-myc的表达情况.结果干扰素（IFN）协同全反式视黄酸（ATRA）可有效地抑制MKN45细胞生长,癌细胞经联合用药诱导后p16和p21基因的表达水平提高,c-myc基因表达水平下降.视黄酸受体RARα基因在MKN45细胞中呈低水平表达,经ATRA和IFN诱导后表达水平提高.结论 ATRA联合IFN诱导可调节p16和p21基因的表达水平,抑制胃癌MKN45细胞生长,这可能与细胞中RARα基因高水平表达有一定关系.
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| 关 键 词： | 全反式视黄酸 干扰素 胃癌细胞系MKN45 |
Influence of Retinoic Acid and Interferon on p16,p21,c-myc Gene Expression in Gastric Carcinoma Cell MKN45 |
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| Authors: | HU Jin-hui LIU Rao SUN Peng ZHU Cong WEI Qing-wen |
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| Institution: | 1.Longgang Central Hospital in Shenzhen City,Shenzhen 518116,China;2.Institute for Drug Control in Shenzhen City,Shenzhen 518114,China;3.People＇s Hospital of Nanshan District in Shenzhen City,Shenzhen 518052,China;4.Pinghu Health Supervision Substation in Longgang District in Shenzhen City,Shenzhen 518111,China) |
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| Abstract: | Objective To evaluate the effect of retinoic acid and interferon on gastric carcinoma cell MKN45.Method To study cells growth and gene p16,p21 and c-myc expression by using MTT,Northern blot and immunohistochemistry methods.Results IFN combined with ATRA can restrain MKN45 growth effectually.Cancer cells induced by the combination,the expression level of p16 and p21 raised,c-myc and RAR descend.Retinoic acid receptor RAR gene in MKN45 cells show low expression,the expression level induced by ATRA and IFN increased.Conclusion ATRA and IFN adjust p16 and p21 expression and inhibit gastric carcinoma cell MKN45 growth,it may correlate with the level of RARα. |
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| Keywords: | all-trans retinoic acid interferon gastric carcinoma cell MKN45 |
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