| 关白附中多糖含量的测定 |
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| 引用本文： | 冯凯,佟海滨,田丹,刘扬,初晓丹,孙新.关白附中多糖含量的测定[J].吉林林学院学报,2009(5):436-438. |
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| 作者姓名： | 冯凯 佟海滨 田丹 刘扬 初晓丹 孙新 |
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| 作者单位： | 北华大学生命科学研究中心,吉林吉林132013 |
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| 基金项目： | 吉林省科技厅基金项目（20080906）. |
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| 摘 要： | 目的采用水提醇沉法提取关白附粗多糖.方法经反复冻融、酶法与Sevag法联合脱蛋白后测定关白附中多糖质量分数.结果多糖经苯酚-硫酸显色后在490 nm处用紫外可见分光光度计进行比色测定,测定结果：关白附中多糖质量分数达4.62%,平均加样回收率为97.72%,RSD=1.96%.结论该方法实验操作简便,准确可靠,重现性较好.
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| 关 键 词： | 关白附 多糖 质量分数测定 硫酸苯酚法 |
Determination of Polysaccharide Content in Aconitum coreanum |
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| Authors: | FENG Kai TONG Hai-bin TIAN Dan LIU Yang CHU Xiao-dan SUN Xin |
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| Institution: | ( Life Science Research Center of Beihua University,Jilin 132013, China) |
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| Abstract: | Aconitum coreanum was extracted with hot water,then the crude polysaccharide fraction was obtained by ethanol precipitation. After the freeze-thaw process,the crude polysaccharide was deproteinated by a combination of proteinase and Sevag method. The polysaccharide content in Aconitum coreanum was determined by phenol-sulfuric acid method comparing colors at 490 nm. Results showed that：the polysachardes content in Aconitum coreanum is 4.62% ,the average recovery rate is 97.72% and RSD is 1.96%. This method is simple,the result is stable and reproducible. |
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| Keywords: | Aconitum coreanum Polysaccharide Determination of the content Phenol-sulfuric acid method |
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