SBHL对HeLa细胞c-myc,H—ras和hTRT基因表达的研究 |
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引用本文: | 艾金霞.SBHL对HeLa细胞c-myc,H—ras和hTRT基因表达的研究[J].吉林林学院学报,2009(4):319-321. |
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作者姓名: | 艾金霞 |
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作者单位: | 北华大学医学检验学院,吉林吉林132013 |
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基金项目: | 吉林市卓依康纳中药研发基金(ZY040301). |
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摘 要: | 目的观察澳洲茄胺盐酸盐对HeLa细胞c-myc,H—ras和hTRT基因表达的影响.方法用鼠抗人c-mycMcAb,H-ras McAb和hTRTPcAb作为一抗,生物素化羊抗鼠IgG作为二抗,用免疫组化方法测定澳洲茄胺盐酸盐对HeLa细胞c-myc,H—ms和hTRT基因的表达.结果澳洲茄胺盐酸盐处理的HeLa细胞与对照组相比,c-myc,H-ras和hTRT基因的表达均明显降低(P〈0.05).结论澳洲茄胺盐酸盐下调HeLa细胞c-myc和H-ras基因的表达,抑制细胞增殖,诱导分化;澳洲茄胺盐酸盐下调HeLa细胞hTRT基因的表达,抑制端粒酶活性,抑制细胞生长.
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关 键 词: | 澳洲茄胺盐酸盐 HeLa细胞 c-myc基因 H—ras基因 hTRT基因 端粒酶 |
Sdosodine Hydrochlorida on Gene Expressions of c-myc,H-ras and hTRT of HeLa Cells |
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Authors: | AI Jin-xia |
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Institution: | AI Jin-xia ( Medical Inspect College of Beihua University, Jilin 132013, China) |
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Abstract: | Objective To observe the effects of SBHL on the expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cells. Methods Mouse anti-human c-myc McAb, H-ras McAb and hTRT polyclonal antibody was used specific antibody and sheep anti-mouse IgG was used the second antibody. The expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cells were detected by immunohistochemistry assy. Results After treated by Sdosodine Hydrochlorida, the expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cell decreased obviously( P 〈 0.05 ). Conclusion The expressions of c-myc gene and H-ras gene were downregulated, the growth of HeLa cell was inhibited and the differentiation of HeLa cell was induced by Sdosodine Hydrochlorida; The expressions of hTRT gene was downregulated, telomerase activity was inhibited and the growth of HeLa cell was inhibited. |
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Keywords: | Sdosodine hydrochloridia HeLa cell c-myc gene H-ras gene hTRT gene Telomerase |
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