水分胁迫下DCPTA对寒地春玉米种子萌发及幼苗生长的影响

本研究以玉米品种郑单958为实验材料,通过设置15%PEG-6000模拟干旱,研究了植物生长调节剂2-(3,4-二氯苯氧基)三乙胺(DCPTA)对寒地春玉米种子萌发和幼苗生长的影响。发芽试验5组处理为:清水(CKA1)、15%PEG-6000溶液(CKA2)、0.5 mg/L DCPTA+15%PEG-6000溶液(A1)、1.0 mg/L DCPTA+15%PEG-6000溶液(A2)、1.5 mg/L DCPTA+15%PEG-6000溶液(A3)。苗期实验5组处理为:清水(CKB1)、15%PEG-6000溶液(CKB2)、20 mg/L DCPTA+15%PEG-6000溶液(B1)、50 mg/L DCPTA+15%PEG-6000溶液(B2)、80 mg/L DCPTA+15%PEG-6000溶液(B3)。发芽实验结果表明,水分胁迫条件下,DCPTA处理促进了种子萌发,使发芽势、发芽率、萌发抗旱指数、胚根干重、胚芽干重、胚根长度、胚芽长度,贮藏物质运转率显著增加,且1.0mg/L DCPTA处理显著改善了种子的萌发特性。盆栽实验结果表明,水分胁迫下玉米幼苗叶片喷施DCPTA增加了玉米幼苗的保护酶活性、叶绿素含量、Fo和Fv/Fm值,并且促进了脯氨酸的积累,5组处理相比大致趋势为清水处理80 mg/L50 mg/L20 mg/L0mg/L,喷施80 mg/L DCPTA处理明显改善了幼苗的植株生长。

Effect of DCPTA on seed germination and seedling growth of spring maize under water stress condition in the cold region of China

To investigate the effects of a plant growth regulator of 2-(3,4-dichloropheNO_xy1)-triethylamino (DCPTA) on the seed germination and the seedling growth of spring maize under drought conditions, Zhengdan 958 was chosen as the experimental material; 15% PEG-6000 was set up to simulate the plant water stress. Treatments of the seed germination experiment were as follows: clean water (CKA1), 15% PEG-6000 solution (CKA2), 0.5 mg/L DCPTA+15% PEG-6000 mixed solution (A1), 1.0 mg/L DCPTA+15% PEG-6000 mixed solution (A2), 1.5 mg/L DCPTA+15% PEG-6000 mixed solution (A3). Treatments of the seedling growth pot experiment were as follows: clean water (CKB1), 15% PEG-6000 (CKB2), 0.5 mg/L DCPTA+15% PEG-6000 (B1), 1.0 mg/L DCPTA+15% PEG-6000 (B2), 1.5 mg/L DCPTA+15% PEG-6000 (B3). The result showed that DCPTA promoted the seed germination, presenting the significant increases of germination potential, germination rate, germination index of drought resistance, the dry weight of radicle and plumule, the length of radicle and plumule and storage substance transportation rate under the condition of water stress; and the 1.0 mg/L DCPTA significantly improved seed germination. The seedling growth pot experiment result showed that the sprays of DCPTA on plants increased the activity of protective enzymes of maize seedlings, the chlorophyll content and the Fo and Fv/Fm values, and promoted the accumulation of proline in plants. It showing a following trend: water >80 mg/L>50 mg/ L>20 mg/L>0 mg/L. The 80 mg/L DCPTA improved the maize seedling growth and development significantly. The outcomes of the present study can provide the theoretical and experimental basis for promoting the germination and seedling growth of spring maize under drought conditions.