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Impact of Bovine Skeletal Muscle Satellite Cell Differentiation by Small Interfering RNA Targeting Myogenin Gene
Authors:Liu  Cong-cong Zhao  Dan-dan Tong  Hui-li Ye  Feng Yang  Yue Li  Shu-feng Jia  Ming-yu Yan  Yun-qin
Affiliation:College of Life Sciences,Northeast Agricultural University
Abstract:To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.
Keywords:myogenin   small interfering RNA   adult bovine skeletal muscle satellite cell   differentiation
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