| Improvement of spergualin production by mutation and medium optimization |
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| 作者姓名： | SHI Yingzi MA Guangpeng WU Xiangjin JIANG Ling WANG Xiangjing* and BAI Hua College of Life Sciences Northeast Agricultural University Harbin China China Rural Technology Development Center Beijing China Zhejiang Hisun Pharmaceutical Co. Ltd. Taizhou Zhejiang China |
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| 作者单位： | SHI Yingzi1，MA Guangpeng2，WU Xiangjin1，JIANG Ling1，WANG Xiangjing1*，and BAI Hua3 1College of Life Sciences，Northeast Agricultural University，Harbin 150030，China 2China Rural Technology Development Center，Beijing 100862，China 3 Zhejiang Hisun Pharmaceutical Co.，Ltd.，Taizhou 318000，Zhejiang，China |
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| 基金项目： | 东北农业大学校科研和教改项目 |
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| 摘 要： | In order to screen a high-yield spergualin-production strain and an optimal fermentation medium,large numbers of isolates were selected after ultraviolet(UV)mutation and self-tolerant mutation of Bacillus laterosporus A7.A high-yield strain A-94-7 was obtained and the spergualin productivity was 4.1-fold of the parent strain.The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good.After fermentation medium optimization,Bacillus laterosporus A-94-7 was able to produce 380 mg?L-1.The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization.
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| 关 键 词： | 精液蛋白 微生物 遗传学 基因突变 |
| 文章编号： | 1006-8104（2007）-04-0310-04 |
| 收稿时间： | 2007-04-10 |
Improvement of spergualin production by mutation and medium optimization |
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| SHI Yingzi,MA Guangpeng,WU Xiangjin,JIANG Ling,WANG Xiangjing*,and BAI Hua College of Life Sciences,Northeast Agricultural University,Harbin ,China China Rural Technology Development Center,Beijing ,China Zhejiang Hisun Pharmaceutical Co.,Ltd.,Taizhou ,Zhejiang,China.Improvement of spergualin production by mutation and medium optimization[J].Journal of Northeast Agricultural University,2007,14(4):310-313. |
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| Authors: | SHI Yingzi MA Guangpeng WU Xiangjin JIANG Ling WANG Xiangjing BAI Hua |
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| Abstract: | In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet (UV) mutation and self-tolerant mutation of Bacillus laterosporus A7. A high-yield strain A-94-7 was obtained and the spergualin productivity was 4.1-fold of the parent strain. The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good. After fermentation medium optimization, Bacillus laterosporus A-94-7 was able to produce 380 mg·L-1. The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization. |
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| Keywords: | spergualin Bacillus laterosporus self-tolerant mutation medium optimization |
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