| 厚朴种源同工酶初步研究 |
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| 引用本文： | 朱玉球,童再康,斯金平.厚朴种源同工酶初步研究[J].浙江农林大学学报,2000,17(1):32-36. |
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| 作者姓名： | 朱玉球 童再康 斯金平 |
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| 作者单位： | 1.浙江林学院 资源与环境系, 浙江临安 311300;2.浙江省景宁县科学技术委员会, 浙江 景宁 323500 |
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| 基金项目： | 九五 浙江省科学技术委员会资助项目(961102111) |
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| 摘 要： | 采集5 年生种源试验林共13 个厚朴种源130 个单株的树叶样品, 用聚丙烯酰胺垂直板凝胶电泳法测定了POD 、EST 和GOT 等3 种酶系同工酶。结果表明:GOT 同工酶各种源仅有1 条谱带, 鄂西种源与其他种源呈等位基因分离关系;POD 和EST 同工酶各有7 条和10条带。经3 种酶系谱带单独分析和综合3 种酶系的相似系数聚类分析, 所划分的类群同叶形分化类型基本一致。图4 表2 参9
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| 关 键 词： | 厚朴 种源试验 同工酶 聚类分析 |
| 收稿时间： | 1999-05-20 |
A Preliminary study on isozymes of Magnolia officinalis from different provenances |
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| ZHU Yu-qiu,TONG Zai-kang,SI Jin-ping.A Preliminary study on isozymes of Magnolia officinalis from different provenances[J].Journal of Zhejiang A&F University,2000,17(1):32-36. |
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| Authors: | ZHU Yu-qiu TONG Zai-kang SI Jin-ping |
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| Institution: | 1.Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;2.Scientific and Technological Commission of Jingning County , Jingning 323500, Zhejiang , China |
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| Abstract: | Leaf samples from 130 individual trees in a 5-year experimental stand belonging to 13 provenances of Magnolia officinalis were collected for an analysis of isozyme systems , vix .POD , EST and GOT by means of SDS-PAGE .The results indicated that the isozymes of GOT for different provenances only had one band and allele segregation exists between the provenance from Hubei and other provenance isozyems of POD and EST had 7 and 10 bands , respectively .Class groups were divided on the basis of separate analysis of the band patterns of the 3 enzyme systems and cluster analysis of similarity coefficients conform with types of leaf shape differentiation . |
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