不同家系马尾松插穗内源生根抑制物的分离、纯化及鉴定

为提高马尾松Pinus massoniana扦插繁殖生根率，采用浸渍法、萃取法和高效液相色谱法（HPLC）对5个家系插穗内源抑制物进行分离提纯，并用HPLC自动积分仪测定了内源抑制物各组分相对含量。结果表明：每个家系插穗内内源抑制物都含有7种组分，它们为脱落酸类、黄酮类和苯酚类物质，但其相对含量在5个家系插穗内存在显著差异（P=0.032 9＜0.05），表明这些内源抑制物质受遗传基因的控制。由内源抑制物对白菜Brassica chinensis幼根生长的影响的方差分析结果可知，内源抑制物有较强的抑制活性，内源抑制物各组分对白菜幼根生长的抑制程度存在极显著差异（P=0.000 1＜0.01）；在5个家系之间，插穗内内源抑制物的同一组分对白菜幼根生长的抑制程度无显著差异（P = 0.088 1＞0.05）。由内源抑制物与生根率之间的相关性可知，插穗内内源抑制物的含量与插穗生根率呈显著性负相关。另外，对分离物和乙醚萃取残余液进行生物鉴定表明，内源抑制物质可分为2类：一类易溶于水的，另一类易溶于乙醚等非极性溶剂。图2表4参11

Separation, purification, and identification of endogenous inhibitory substances in Pinus massoniana cuttings

To improve the rooting rate of Pinus massoniana, endogenous inhibitors from cuttings of five P. massoniana lines were purified and separated using extraction and high pressure liquid chromatography （HPLC）. Biological assays along with analysis of variance （ANOVA） with 4 treatments of aether extracts and 3 replications with comparisons to Brassica chinensis seeds were employed. Results revealed seven components of endogenous inhibitors in each P. massoniana cutting belonging to flavones, phenols, and abscisic acid（ABA）. This indicated that their expression was controlled by genes with significant differences among lines （P = 0.032 9）. By comparison, an ANOVA for the influence of endogenous inhibitors on growth of B. chinensis roots was highly significant （P = 0.000 1）; whereas compared to the same component of endogenous inhibitors in the root growth of B. chinensis, inhibition with the five P. massoniana lines were not significantly different. Also, except for MI6 （No. 6 of HLPC educts）, correlation analyses between the relative content of endogenous inhibitors and the rooting rate of B. chinensis were negative. Biological assays for extractions and their residuals extracted with ether showed two kinds of endogenous inhibitors： one thatdissolved easily in water and the other that was easily soluble in a non-polar solvent.