| 泰国巨竹试管快繁研究 |
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| 引用本文： | 周玲,王晓芹,林新春.泰国巨竹试管快繁研究[J].浙江林学院学报,2014(5):817-820. |
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| 作者姓名： | 周玲 王晓芹 林新春 |
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| 作者单位： | 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 |
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| 基金项目： | 国家自然科学基金资助项目（31270677）;“十二五”浙江省农业新品种选育“竹木育种协作组”课题（2012C12908-3）;浙江省自然科学基金重点资助项目（Z3100366）;浙江省优先主题重点农业项目（2010C12011） |
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| 摘 要： | 以泰国巨竹Gigantochloa tekserah成熟种胚为材料，研究不同激素组合对泰国巨竹丛生芽增殖及生根的影响，建立其试管繁殖体系。结果表明：泰国巨竹丛生芽的最佳增殖培养基为MS（Murashige and Skoog）＋3 mg·L^-1BA（6-苄基腺嘌呤）＋0．01 mg·L^-1TDZ（噻苯隆）＋0.3 mg·L^-1NAA（萘乙酸），增殖系数为2．11，芽丛生长旺盛；最优生根培养基为1／2MS＋3．0 mg·L^-1IBA（吲哚丁酸），生根率达70％，根系发达、粗壮；试管苗移栽至等配比的泥炭、蛭石、珍珠岩的基质中，成活率达100％。
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| 关 键 词： | 植物学 泰国巨竹 组织培养 丛生芽 植物生长调节物质 |
In vitro micropropagation of Gigantochloa tekserah |
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| ZHOU Ling,WANG Xiaoqin,LIN Xinchun.In vitro micropropagation of Gigantochloa tekserah[J].Journal of Zhejiang Forestry College,2014(5):817-820. |
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| Authors: | ZHOU Ling WANG Xiaoqin LIN Xinchun |
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| Institution: | (The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A ＆ F University, Lin＇an 311300, Zhejiang, China) |
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| Abstract: | To establish a tissue culture system for commercial propagation, buds induced by mature embryos were used as explants to study the effect of different hormone combinations on bud muhiplieation and root in- duction of Gigantochloa tekserah. Results showed that for bud multiplication, since the highest proliferation co- efficient was 2.11 and cluster buds grew well, Murashige and Skoog＇s （MS） medium supplemented with 3 mg.L^-1 6-benzylaminopurine （BA）, 0.01 mg.L^-1 thidiazuron （TDZ）, and 0.3 mg.L^-1 1-naphthylaeetie acid （NAA） was the optimal medium. The best rooting medium was 1/2 MS supplemented with 3 mg.L^-1 indole-3-butyrie acid （IBA） because of the rooting rate was up to 70% with long, thick roots. After transferring to an equal ratio mixture of peat, vermiculite, and perlite, the survival rate of hardening plantlets was 100%. |
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| Keywords: | botany Gigantochloa tekserah tissue culture clustered shoots plant growth regulator |
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