菲白竹组织培养技术

以菲白竹Sasafortunei幼嫩竹秆和竹鞭为材料，研究其组织培养快繁关键技术。结果表明：以竹鞭为外植体萌芽率较高，添加6-苄基腺嘌呤（6-BA）试管苗增殖效果优于噻二唑苯基脲（TDZ），但TDZ能促进新芽生根；以MS（Murashige and Skoog）为基本培养基，分别添加3mg·L^-1 6-BA与0．5mg·L^-1萘乙酸（NAA），侧芽增殖系数达3．60；添加3mg·L^-1 6-BA与0．01mg·L^-1 TDZ，增殖系数较高（3．43），且伸长生长迅速，生根率达到100％，可同时实现增殖与生根，有利于菲白竹大规模快速繁殖。图1表3参9

Micropropagation of Sasa fortunei

Micropropagation of Sasa The results showed as follows： fortunei was studied with shoots of young culms and rhizomes as explants, germination rate of rhizomes was better than that of culms. 6- Benzylaminopurine （6-BA） had a better effect on proliferation than thidazuron （TDZ）. But TDZ was able to improve rooting. The highest proliferation rate （3.60） was obtained from the combination of 3 mg·L^-1 6-BA and0.5 mg ·L^-1 NAA. The combination of 3 mg·L^-1 6-BA and 0.01 mg·L^-1TDZ also had a high proliferation rate （3.43）, achieving 100% rooting percentage and good growth performance. Therefore, it was potentially propitious to mass production. Ch, 1 fig. 3 tab. 9 ref.]