诱导型启动子Rd29A引导的植物表达载体的构建

全球20%的耕地和近半数的灌溉土地都受到不同程度的盐害威胁,抗盐育种具有重要意义.针对常规育种周期长等明显的局限性,利用抗盐转基因可以迅速获得抗盐材料,明显缩短育种周期.利用PCR技术克隆获得Rd29A这一干旱、盐碱等逆境诱导表达的特异性启动子,与叶绿体转运肽和甜菜碱醛脱氢酶基因构建pBinRdMCS重组质粒,用于林木转化.通过克隆Rd29A启动子构建逆境诱导的高效表达载体,可对林木抗盐转基因育种提供理论指导. 图5参8

Rd29A gene cloning and plant expression vector constructed

It was global problem that effected of saline on forest and agriculture soil,about 20% plowlands and half of irrigated soils had been affected by saline.It's important to speeded salt-tolerance breeding course.Transgenic breeding can fasted the salt-tolerance pace,and shorten breeding cycle significantly.So we designed the primer and cloning Rd29A promoter by PCR,constucted pBinRdMCS plasmid that BADH gene can be induced by salt and drought,then it was transit to chloroplast.The results analysis by PCR and restriction show that the plasmid include aim gene.The plasmid will be used for tree transgenic breeding.