PEG6000渗透处理对油松种子发芽的影响

通过正交试验探讨聚乙二醇（PEG 6000）溶液渗透处理对油松Pinus tabulaeformis种子萌发的影响。利用不同处理组合（PEG 6000溶液质量分数,处理时间和处理温度）分别对2002年不同含水量种子和2004年人工老化不同时间的种子进行预处理,并进行棉床发芽试验,对种子发芽率、发芽指数和活力指数进行统计分析,并筛选出最佳处理组合。综合各指标获得以下最佳处理组合,2002年种子：含水量9.84%和6.03%＋200 g.kg-1PEG 6000＋处理时间1d;2004年种子：人工老化0 d＋200 g.kg-1PEG 6000＋处理温度25℃＋处理时间2 d。种子发芽率较高,发芽较整齐。PEG 6000溶液渗透处理可以促进油松种子萌发,溶液质量分数、处理时间和处理温度对处理效果具有明显影响;油松种子发芽的适宜含水量范围较大,但含水量太低则会抑制种子萌发;人工老化可以模拟自然老化种子活力变化,PEG渗透处理有利于老化种子恢复活力。

Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments

The effects of polyethylene glycol （PEG） 6000 osmotic treatments on germination of Pinus tabulaeformis seeds were studied through an orthogonal design （L9 3^4, L16 4^5）. Seeds harvested in 2002 had differing water content, and seeds harvested in 2004 were artificially aged （40 ℃, 100% RH） to different time periods. These seeds were then subjected to different pretreatment combinations of PEG solution level, treatment duration, and temperature. Next, seeds were germinated on a cotton bed, and the germination rate, germination index （ G1 = ∑G1 （germination rate in certain days）/Dt （ number of germination days） ） , and vigor index （ V1 = G1/fresh weight of seedling） were statistically analyzed. Seeds harvested in 2002 had a water content of either 9. 84% or 6.03% , were treated with a 200 g ·kg -1 PEG solution, and had a treatment time of 1 d; seeds harvested in 2004 were artificially aged 0 d, were treated with a 200 g ·kg -1 PEG solution, had a treatment temperature of 25℃ and a treatment duration of 2 d. The seed germination rate was higher （ P 〈 0. 05 ） after treatment and seeds sprouted synchronously. The range of water content for seed germination was wide with seed germination being inhibited at lower seed water content. Seed vigor decreases due to natural aging that were simulated with artificial aging were restored with PEG treatment of the artificially aged seeds. The different content, treatment duration, and temperature of the PEG solution had a definite influence on the seeds with the PEG treatments, overall, helpful for Chinese pine seed germination. Ch, 4 tab. 14 ref. ]