| 银杏核糖体DNA内转录间隔序列初步分析 |
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| 引用本文： | 桂仁意,金爱武,高培军,曹福亮.银杏核糖体DNA内转录间隔序列初步分析[J].浙江林学院学报,2007,24(1):17-21. |
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| 作者姓名： | 桂仁意 金爱武 高培军 曹福亮 |
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| 作者单位： | 1. 浙江林学院,林业与生物技术学院,浙江,临安,311300
2. 南京林业大学,森林资源与环境学院,江苏,南京,210037 |
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| 摘 要： | 为研究银杏Ginkgo biloba种内核糖体DNA内转录间隔（ITS）区序列的多样性，以具有代表性的10个银杏嫩叶样本为材料，提取基因组DNA后用特异性引物进行PCR扩增，并直接测定其ITS区序列。结果表明，银杏ITS区（内含5．8S）总长度为1224～1226bp，其中ITS-1长度为821—823bp，5．8S长度为162bp，ITS-2长度为241bp。在全序列范围内，可变位点有28个，占总核苷酸的2．3％，但简约信息位点仅6个，变异量仅有0．5％。银杏的不同样本之间ITS区序列表现出高度一致。
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| 关 键 词： | 植物学 银杏 内转录间隔 序列测定 |
| 文章编号： | 1000-5692（2007）01-0017-05 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006-03-272006-10-08 |
Internal transcribed spacer (ITS) sequences with Gingko biloba populations |
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| GUI Ren-yi,JIN Ai-wu,GAO Pei-jun,CAO Fu-liang.Internal transcribed spacer (ITS) sequences with Gingko biloba populations[J].Journal of Zhejiang Forestry College,2007,24(1):17-21. |
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| Authors: | GUI Ren-yi JIN Ai-wu GAO Pei-jun CAO Fu-liang |
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| Abstract: | The diversity of nuclear ribosomal DNA(rDNA)internal transcribed spacer(ITS) sequences of Ginkgo biloba was determined by analyzing 10 leaf samples resenting different populations.Results showed that the total length of the ITS sequences,including 5.8 S,was 1 224-1 226 base pairs(bp)with ITS-1 being 821-823 bp long,5.8 S having 162 bp,and ITS-2 consisting of 241 bp.Among the sequences,there were 28 variable sites,or 2.3% of the total,but only 6 parsim-informative sites,or 0.5% of the total.The ITS sequences among the different populations were very similar.Ch,2 fig.13 ref.] |
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| Keywords: | botany Ginkgo biloba internal transcribed spacer(ITS) sequencing |
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