| 菊属植物花粉生活力检测方法的比较 |
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| 引用本文： | 赵宏波,陈发棣,房伟民.菊属植物花粉生活力检测方法的比较[J].浙江林学院学报,2006,23(4):406-409. |
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| 作者姓名： | 赵宏波 陈发棣 房伟民 |
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| 作者单位： | 南京农业大学,园艺学院,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金;江苏省高校高新技术产业发展项目;江苏省科技厅高新技术基金;上海市科技兴农科技攻关项目 |
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| 摘 要： | 利用碘-碘化钾法、氯化三苯基四氮唑(TTC)法及改进TTC法、无机酸法、荧光染色法、离体萌发法、活体萌发法测定了菊属Dendranthema植物菊花脑Dendranthemanankingense,南京野菊Dendranthema indicum,毛华菊Dendranthema vestitum和栽培小菊品种rm43-2,rm46-1,rm48-2的花粉生活力。结果表明:碘-碘化钾法、TTC法及改进TTC法、无机酸法和荧光染色法都不适合菊属植物花粉生活力的检测;活体萌发法检测菊属植物花粉生活力不准确,但能有效表述花粉萌发力;离体萌发法能有效快速地测定花粉生活力,适合菊属植物花粉生活力的检测。图1参11
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| 关 键 词： | 植物学 栽培小菊 菊属植物 花粉活力 检测方法 |
| 文章编号： | 1000-5692（2006）04-0406-04 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2006-01-06 |
Detection methods of pollen viability of Dendranthema |
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| ZHAO Hong-bo,CHEN Fa-di,FANG Wei-min.Detection methods of pollen viability of Dendranthema[J].Journal of Zhejiang Forestry College,2006,23(4):406-409. |
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| Authors: | ZHAO Hong-bo CHEN Fa-di FANG Wei-min |
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| Institution: | College of Horticulture, Nanjng Agricultural University, Nanjing 210095, Jiangsu, China |
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| Abstract: | The pollen viability of Dendranthema indicum,D.vestitum,D.nankingense and three cultivars of chrysanthemum with small inflorescences(rm43-2,rm46-1,rm48-2)were detected by methods of iodine-iodide kalium,triphenyltetrazolium chloride(TTC)or modified TTC,FDA,mineral acid,germination in vitro and in vivo.The results showed that the methods of iodine-iodide kalium,TTC or modified TTC,FDA,mineral acid were not able to detect the pollen viability of chrysanthemum.The method of germination in vivo can not test the pollen viability but describe the pollen vigor.The method of germination in vitro was fit for detection of pollen viability of chrysanthemum. |
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| Keywords: | botany chrysanthemum cultivars with small inflorescences Dendranthema pollen viability detection |
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