| 关中马mtDNA D-loop区遗传多态性分析 |
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| 引用本文： | 苏锐,谢文美,张云生,王小斌,张春梅,王美菊,陈宏,雷初朝.关中马mtDNA D-loop区遗传多态性分析[J].西北农业学报,2008,17(6):17-20. |
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| 作者姓名： | 苏锐 谢文美 张云生 王小斌 张春梅 王美菊 陈宏 雷初朝 |
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| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100
2. 西北农林科技大学,动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100;徐州师范大学,细胞与分子生物学研究所,江苏徐州,221116 |
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| 基金项目： | 教育部新世纪优秀人才支持计划
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西北农林科技大学科研专项基金
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| 摘 要： | 利用测序技术和生物信息学分析技术,对关中马27个个体的线粒体DNA D-loop 247 bp序列的遗传多态性及系统进化进行分析,检测到9种单倍型,其核苷酸多态位点19个,约占所测核苷酸总长的7.69%,均为转换。关中马mtDNA D-loop区核苷酸多样度(π值)为0.0219±0.0076,单倍型多样度(H)为0.843±0.041,表明关中马mtDNA遗传多态性丰富。根据mtDNA序列构建了关中马的NJ分子系统树,发现存在A、C、D、F和G共5个支系,表明关中马是多起源的。
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| 关 键 词： | 关中马 多态性 单倍型 支系 |
| 收稿时间： | 2008/5/15 0:00:00 |
| 修稿时间： | 2008/7/18 0:00:00 |
Analyses on mtDNA D-loop Genetic Diversity in Guanzhong Horse |
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| SU Rui,XIE Wen-mei,ZHANG Yun-sheng,WANG Xiao-bin,ZHANG Chun-mei,WANG Mei-ju,CHEN Hong and LEI Chu-zhao.Analyses on mtDNA D-loop Genetic Diversity in Guanzhong Horse[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(6):17-20. |
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| Authors: | SU Rui XIE Wen-mei ZHANG Yun-sheng WANG Xiao-bin ZHANG Chun-mei WANG Mei-ju CHEN Hong and LEI Chu-zhao |
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| Institution: | College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou Jiangsu 221116, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China |
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| Abstract: | The genetic diversity and phylogenetic evolution of 247 bp mitochondrial DNA(mtDNA) Dloop sequences of 27 Guanzhong horse samples were analyzed by sequencing and bioinfo rmation tech niques.The results revealed 9 mitochondrial haplot ypes with 19 poly morphic sites,covering 7.69% of the analyzed length of the 247 bp Dloop sequences.A mong the polymor phicsites,there were all transitions.The nucleotide diversity(value) and haploty pe diversity(H) estimated were 0.0219 0.0076 and 0.843 0.041,respectively,showing high mitochondrial genetic diversity in Guanzhong horse breed.The const ructed Neighbor Joining molecular phylog enetic tree based on the 9 m tDNA Dloop haploty pes,indicated that lineages A,C,D,F and G were detected which showed multiple maternal origins in Guanzhong horse breed. |
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| Keywords: | mtDNA D-loop |
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