| 利用SSR和InDel标记构建白菜×芜菁分子遗传图谱 |
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| 引用本文： | 赵 湘,于拴仓,薛林宝,张凤兰,赵 凡,余阳俊,赵岫云,张德双,汪维红.利用SSR和InDel标记构建白菜×芜菁分子遗传图谱[J].西北农业学报,2011,20(12):111-115. |
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| 作者姓名： | 赵 湘 于拴仓 薛林宝 张凤兰 赵 凡 余阳俊 赵岫云 张德双 汪维红 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心;扬州大学园艺与植物保护学院 |
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| 基金项目： | 北京市自然科学基金项目(6102011);农业部公益性行业科研专项(nyhyzx07-007);北京市科技计划项目(D11110000131102);国家科技支撑项目(2009BADB8B03) |
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| 摘 要： | 以结球白菜BY和芜菁MM为亲本建立的小孢子培养DH系作为图谱构建群体,利用筛选获得的86对SSR引物和66对InDel引物进行分离分析,并整合已有的55个SSR标记,构建包含10个连锁群、175个标记位点的分子连锁图谱,图谱总长度902.9cM,标记间的平均图距为5.7cM,每个连锁群上的标记数为9～28个,平均图距为3.2～8.8cM,连锁群长度为66.0～113.3cM。
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| 关 键 词： | 大白菜 芜菁 遗传图谱 SSR InDel |
Construction of a Genetic Linkage Map in Chinese Cabbage×Turnip Based on SSR and InDel Markers |
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| ZHAO Xiang,YU Shuancang,XUE Linbao,ZHANG Fenglan,ZHAO Fan,YU Yangjun,ZHAO Xiuyun,ZHANG Deshuang and WANG Weihong.Construction of a Genetic Linkage Map in Chinese Cabbage×Turnip Based on SSR and InDel Markers[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(12):111-115. |
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| Authors: | ZHAO Xiang YU Shuancang XUE Linbao ZHANG Fenglan ZHAO Fan YU Yangjun ZHAO Xiuyun ZHANG Deshuang and WANG Weihong |
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| Institution: | 1(1.Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China; 2.Horticulture and Plant Protection College of Yangzhou University,Yangzhou Jiangsu 225009,China) |
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| Abstract: | A DH population derived from the F1 between Chinese cabbage BY and Turnip MM was employed to construct the genetic linkage map based on SSR and InDel markers.86 SSR and 66 InDel markers showed polymorphism and were analyzed in the DH population,in addition,55 previously mapped SSR markers were also used in mapping.175 markers were integrated into 10 groups.It covered a total of 902.9 cM with an average interval of 5.7 cM.The number of markers in each linkage group varied from 9 to 28,the length from 66.0 cM to 113.3 cM and the average interval from 3.2 cM to 8.8 cM.The molecular genetic map will provide basis for gene localization in Chinese cabbage and turnip. |
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| Keywords: | Chinese cabbage(Brassica rapa ssp pekinensis) Turnip(B rapa ssp rapifera) Genetic linkage map SSR InDel |
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