过表达GhMYB4基因对棉花茎秆木质素合成的影响

旨在探究过表达陆地棉(Gossypiumhirsutum)GhMYB4基因对棉花茎秆木质素合成的影响。利用Wiesner组织化学染色的方法对棉花茎秆进行染色,并用Klason法对棉花茎秆的局部位置进行木质素总量测定。对距离下胚轴0～1 cm、2～7cm、10～12 cm处不同茎秆部位的次生壁木质素进行化学组织染色,定量分析染色组织结构发现,大部分的转基因株系相比对照受体株系在初生木质部和初生韧皮部的染色范围、细胞层数和染色程度上均有一定的显著性增加。通过对29个过表达转基因株系和对照受体株系在棉花茎秆距离下胚轴2～7cm处木质素总量的定量分析发现,有23个过表达转基因株系相比于对照受体株系的木质素总量有显著性的增加和1个株系出现显著性降低的现象。这些结果表明GhMYB4基因参与调控棉花茎秆的木质素生物合成。

Effects of Overexpression of GhMYB4 Gene on Lignin Synthesis in Cotton Stem

his paper aims to investigate the function of GhMYB4 gene in Gossypium hirsutum and analyze its regulation of lignin synthesis during overexpression and development.Cotton stalks were stained by Wiesner histochemical staining method,and the total lignin content of cotton stalks was determined by Klason method according to the degree of dyeing.By chemically staining the secondary wall lignin at different stems at 0-1 cm,2-7 cm,and 10-12 cm from the hypocotyl,and comparing the quantitative analysis of the stained tissue structure,most of the results were found.The transgenic lines showed a significant increase in the staining range,cell layer number and staining degree of the primary xylem and the primary phloem compared with the control recipient strain.Quantitative analysis of the total amount of lignin at the 2-7 cm hypocotyl of cotton stalks by 29 overexpressing transgenic lines and control recipient strains revealed that there were 24 overexpressing transgenic lines related to control receptors.The total amount of lignin in the strain increased significantly.The GhMYB4 gene was involved in the regulation of lignin biosynthesis in stems.