甲胺磷降解菌响应曲面法优化研究

从受甲胺磷污染的土壤中分离筛选出甲胺磷降解菌,采用响应曲面法对影响降解菌的5个因素培养温度(X1)、pH值(X2)、甲胺磷的浓度(X3)、装液量(X4)、接种量(X5)的最佳水平范围进行了研究。通过对二次多项回归方程求解得知,在上述自变量取值分别为:温度28℃,pH值7.0,甲胺磷浓度1 500 mg/L,装液量100 mL,接种量10%时,甲胺磷降解率为最大,预测值为73.65%。

Methamidophos-degrading Bacteria for the Extracellular Polysaccharide Fermented by Using Response Surface Methodology

One of methamidophos-degrading bacteria was isolated from polluted soils.Response surface methodology(RSM) based on a five-level five-factor central composite rotatable design of experiments was used to optimize the optimal levels of five important factors,namely temperature(X₁),pH value(X₂),concent ration of methamidophos(X₃),the medium volume(X₄),the quantity of inoculum (X₅).Using the quadratic regression model,the optimum concentrations were determined: temperature 28,pH value 7.0,concent ration 1 500 mg/L,medium volume 100 mL and inoculum amount 10%,respectively,the predicted yield of the methamidophos degrading rate was 73.65%.