ELISA检测蜂王浆氯霉素残留的快速前处理方法

为获得一种能快速检测蜂王浆中氯霉素残留的前处理方法,采用改良的0.1mol/L Tris-TBE(pH=8.0)溶液处理蜂王浆,结合乙酸乙酯提取氯霉素(CAP),处理时间约25min。ELISA检测样品的OD450值明显低于试剂盒法,通过添加标准CAP溶液对改良法进行验证。结果表明,改良法提取的样品对不同CAP质量浓度表现出明显的OD450值变化,与添加前比较差异显著(P<0.05),排除处理过程存在干扰显色的因素。改良法检测相同样品的平均相对标准偏差为1.29%,完全符合目前国际上氯霉素残留检测的要求。

A Quick Pretreatment Method for Determination of Chloramphenicol Residue in Royal Jelly by ELISA

We used the improved method(0.1 mol/L Tris-TBE) to deal with royal jelly samples,then extracted chloramphenicol(CAP) with ethyl acetate.The whole processing only needs 25 min.Compare with the original method from the ELISA kit the OD450 values of the improved method were lower than those of the kit method.We added standard CAP solution to the samples treated by the improved method to verify the sensitivity of it.The OD450 value changed which were obviously different from samples without adding CAP(P<0.05).It was indicated that there was no interfering agent in the process of pretreatment and coloration.The average RSD of enzyme-marked board is 1.29%,wich is accord with the request of international determination of chloramphenicol residue.Our assay provides a quick pretreatment method to detect the CAP in royal jelly.It is useful in the high-throughput detection for the inspection and quarantine departments.