| 孔雀石绿胶体金快速检测试剂板的研制及应用 |
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| 引用本文： | 桑丽雅,王振国,柳爱春.孔雀石绿胶体金快速检测试剂板的研制及应用[J].西北农业学报,2012,21(6):186-191. |
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| 作者姓名： | 桑丽雅 王振国 柳爱春 |
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| 作者单位： | 1. 杭州南开日新生物技术有限公司,杭州,310022
2. 杭州市农业科学研究院,杭州,310024 |
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| 摘 要： | 为建立孔雀石绿免疫胶体金快速检测试剂板,采用杭州南开日新生物技术有限公司实验室自制的包被抗原孔雀石绿-卵清蛋白偶联物(MG-OVA)、抗孔雀石绿单抗作为原料,将各组分经过调试优化后组装成试剂板。结果表明,该试剂板对阴性水产样品的检测结果与液相-串联质谱法(LC-MS/MS)完全符合,对水产品中孔雀石绿和隐性孔雀石绿的最低检出限分别为1μg/kg和3μg/kg,整个检测所需时间约30min。该试剂板具有快速、灵敏、准确的特点。
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| 关 键 词： | 孔雀石绿 胶体金 人工抗原 单克隆抗体 快速检测 |
Development and Application of Colloidal Gold Rapid Test Device for Malachite Green |
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| SANG Liy,WANG Zhenguo and LIU Aichun.Development and Application of Colloidal Gold Rapid Test Device for Malachite Green[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(6):186-191. |
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| Authors: | SANG Liy WANG Zhenguo and LIU Aichun |
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| Institution: | 1.Hangzhou Nankai Biotech Co.,Ltd.,Hangzhou 310022,China; 2.Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China) |
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| Abstract: | Malachite green(MG) colloidal gold rapid test device is mainly made from MG-OVA developed by us,and anti-MG monoclonal antibody with the optimized mixture ratio.Testing results show that the rapid test device and LC-MS/MS have the consistent results to test negative seafood samples the lowest detection limits for MG and leuco-MG are respectively 1 μg/kg and 3 μg/kg,and the testing time for one sample is about 30 minutes.This test device is rapid,sensitive and accurate. |
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| Keywords: | Malachite green Colloidal gold Artificial antigen Monoclonal antibody Rapid test |
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