川楝树皮中川楝素质量分数的液相色谱检测

建立液相色谱仪快速检测川楝树皮中川楝素质量分数的方法。最佳提取条件为甲醇体积分数70%、回流温度70℃、回流时间1h;色谱条件为甲醇与水的混合物V(甲醇)∶V(水)=65∶35)]作流动相,检测波长215nm,流速1.0mL/min,柱温25℃,进样量5μL;川楝素得率2.81‰,添加水平为0.75～3.00mg时,回收率为97.33%～99.33%。该方法能准确快速检测样品川楝素的质量分数。

Determination of Toosendanin in the Bark of Melia Toosendan Sieb. et Zuccc. by High Performance Liquid Chromatography

A reversed-phase high performance liquid chromatographic method was developed for determining toosendanin in the bark of Melia toosendan Sieb.et zuccc.The optimum extraction condition for toosendanin was as following: Volume fraction of methanol 70%,extraction temperature 70 ℃,extraction time 1 h.The method for HPLC assay was with methanol-water V(methanol)∶V(water)=65∶35] as mobile phase and ultraviolet detector at 215 nm.The column temperature was 25 ℃ and the flow rate was 1 mL/ min,the injection volume was 5ul.The yield of toosendanin is 2.81‰.The recoveries at spiked concentrations of 0.75-3.00 mg ranged from 97.33% to 99.33%.It is showed this method is simple,accurate and can be used for determining toosendanin in the bark of Melia Toosendan Sieb.et Zucc.