苦荞麦毛状根的诱导研究

利用发根农杆菌Ri1601浸染苦荞植物预培养2d的叶片外植体,经不同时间的共培养和除菌培养后获得毛状根。经硅胶薄层色谱板检测表明,发根农杆菌Ri1601和苦荞叶片上未经转化产生的不定根都不含有冠瘿碱;而经发根农杆菌Ri1601遗传转化后所产生的毛状根有冠瘿碱的存在。因此,可确定苦荞叶片上产生的毛状根为发根农杆菌Ri1601转化所得。建立了用发根农杆菌Ri1601诱导苦荞植物叶片产生毛状根的有效方法。

Study on Hairy roots Induction of Fagopyrum tataricum Gaertn

The Hairy-roots can be obtained by inoculating two-day-precultured leaf explants of Fagopyrum tataricum Gaertn with Agrobaterium rhizogenes Ri1601.The silicon thin layer chromatography(TLC) tests showed that the adventitious roots produced by leaves of Fagopyrum tataricum Gaertn and Agrobaterium rhizogenes Ri1601 do not contain crown gall alkali.On the other hand,the hairy roots which were produced by Agrobaterium rhizogenes Ri1601-mediated genetic transformation of Fagopyrum tataricum Gaertn leaves have crown gall alkali.Therefore,it can be determined that the hairy roots from leaves of Fagopyrum tataricum Gaertn were produced by Agrobaterium rhizogenes Ri1601-mediated transformation.Due to these,the different factors were designed to examine for their effects on hairy-roots induction and growth.The effective methodology of hairy-roots induction by Agrobaterium rhizogenes was established and provided a foundation for industrial production of active components from Fagopyrum tataricum Gaertn.