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中国野生葡萄染色体倍性研究
引用本文:孙马,王跃进.中国野生葡萄染色体倍性研究[J].西北农业学报,2006,15(6):148-152.
作者姓名:孙马  王跃进
作者单位:西北农林科技大学园艺学院,陕西杨凌,712100
摘    要:利用去壁低渗染色体计数法及流式细胞术法,首次系统地对中国野生葡萄18个种64个株系的染色体倍性进行了鉴定。结果表明,供试的中国野生葡萄全部为二倍体(2n=38)。同时也证明流式细胞仪不仅可以进行染色体倍性的鉴定,且制样简单、测定快速、结果可靠。

关 键 词:中国野生葡萄  染色体数目  流式细胞仪  倍性
文章编号:1004-1389(2006)06-0148-05
收稿时间:2006-07-13
修稿时间:2006-08-10

Study on the Chromosome Ploidy of Chinese Wild Vitis Species
SUN Ma and WANG Yue-jin.Study on the Chromosome Ploidy of Chinese Wild Vitis Species[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(6):148-152.
Authors:SUN Ma and WANG Yue-jin
Institution:College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China
Abstract:Ploidy of 64 clones of 18 wild Chinese wild Vitis species were studied by means of the wall degradation hypotonic method and Flow Cytometry in order to get basic information about the inheritance,variance,evolution and variety character of Chinese wild Vitis.The results showed that all of the 64 observed clones were diploids.The results also showed that Flow Cytometry could be used for identifying ploid and was the ideal method because of the simple preparation of sample,rapid and accurate measurement.
Keywords:Chinese wild Vitis  Chromosome numbers  Flow Cytometry  Ploidy
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