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| 黄瓜子叶节离体再生过程中内源激素变化 | |
| 引用本文： | 张守杰,王秀峰,杨小华,杨凤娟,田红梅.黄瓜子叶节离体再生过程中内源激素变化[J].西北农业学报,2011,20(5):153-157. |
| 作者姓名： | 张守杰 王秀峰 杨小华 杨凤娟 田红梅 |
| 作者单位： | 1. 山东农业大学园艺科学与工程学院,山东泰安,271018 2. 山东农业大学园艺科学与工程学院,山东泰安,271018;作物生物学国家重点实验室,山东泰安,271018 3. 济宁农业科学院,山东济宁,272000 |
| 摘 要： | 采用酶联免疫吸附法(ELISA),测定黄瓜子叶节再生过程中不同发育阶段愈伤组织的内源激素含量,探讨子叶节再生过程中内源激素的变化规律和激素间的相互关系.结果表明,随着黄瓜子叶节再生阶段的变化,内源激素含量也呈现不同变化.外植体培养0 d时,ABA含量最高,IAA和GA含量最低;愈伤组织诱导期(3～9 d),ABA、IAA、GA、ZR含量先升高后降低;愈伤组织分化期(9～15 d),IAA和GA含量均先升高后降低,ZR含量一直降低,而ABA含量则先降低后升高. |
| 关 键 词： | 黄瓜 子叶节 内源激素 组织培养 |
Variation of Endogenous Phytohormone Concentration in Cotyledonary Node Calli during Cucumber Regeneration |
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| ZHANG Shoujie,WANG Xiufeng,YANG Xiaohu,YANG Fengjuan and TIAN Hongmei.Variation of Endogenous Phytohormone Concentration in Cotyledonary Node Calli during Cucumber Regeneration[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(5):153-157. | |
| Authors: | ZHANG Shoujie WANG Xiufeng YANG Xiaohu YANG Fengjuan and TIAN Hongmei |
| Institution: | ZHANG Shoujie1,WANG Xiufeng1,2,YANG Xiaohua3,YANG Fengjuan1,2 and TIAN Hongmei1(1.College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an Shandong 271018,China,2.State Key Laboratory of Crop Biology,3.Jining Agriculture Academy of Sciences,Jining Shandong 272000,China) |
| Abstract: | To investigate the variation of endogenous hormones concentration and their relationship at the different stages of cucumber regeneration,we measured the endogenous abscisic acid(ABA),indole acetic acid(IAA),zeatin riboside(ZR) and gibberellin(GA) in cucumber cotyledonary node calli by Enzyme-Linked Immunosorbent Assay(ELISA).The results showed that the highest content of ABA and the lowest content of IAA and GA were occurred at 0 day after explant culture;at callus induction stage(3-9 d),these endogenous p... |
| Keywords: | Cucumber Cotyledonary node Endogenous phytohormone Tissue culture |
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