| 农杆菌介导TLPD34基因的玉米遗传转化及检测 |
| |
| 引用本文： | 王 琨,胡银岗,杨政伟,张仁和,徐书举,薛吉全.农杆菌介导TLPD34基因的玉米遗传转化及检测[J].西北农业学报,2011,20(5):74-78. |
| |
| 作者姓名： | 王 琨 胡银岗 杨政伟 张仁和 徐书举 薛吉全 |
| |
| 作者单位： | 西北农林科技大学农学院,陕西杨凌,712100 |
| |
| 基金项目： | 陕西省农业攻关项目资助 |
| |
| 摘 要： | 以玉米优良自交系郑58为受体材料,将抗真菌病害的水稻类甜蛋白基因(TLPD34)转入玉米,获得451株转化植株,经200mg/L草胺磷有效质量浓度筛选,得到57株抗性苗,T0代检测后,最终获得13株阳性植株,转化率达2.9%。对T0代PCR检测呈阳性的植株进行RT-PCR发现,部分植株能够进行正常转录。表明该方法能将外源基因TLPD34整合到玉米自交系郑58基因组中并进行正常转录。
|
| 关 键 词： | 玉米 茎尖 农杆菌介导 TLPD34 |
Studies of Transformation of TLPD34 Gene into Maize by Agrobacterium Mediated and Detection |
| |
| WANG Kun,HU Yingang,YANG Zhengwei,ZHANG Renhe,XU Shuju and XUE Jiquan.Studies of Transformation of TLPD34 Gene into Maize by Agrobacterium Mediated and Detection[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(5):74-78. |
| |
| Authors: | WANG Kun HU Yingang YANG Zhengwei ZHANG Renhe XU Shuju and XUE Jiquan |
| |
| Institution: | WANG Kun,HU Yingang,YANG Zhengwei,ZHANG Renhe,XU Shuju and XUE Jiquan(College of Agronomy,Northwest A&F University,Yangling Shaanxi 712100,China) |
| |
| Abstract: | The TLPD34 gene was transferred into the shoot apical point of maize inbred line zheng 58 by Agrobacterium-mediated transformation.We have obtained 451 transgenic plants in all,57 transgenic plants were obtained after selecting with 200 mg/L glufosinate and thirteen positive transgenic plants were confirmed by PCR.The frequency of transformation reached about 2.9%.Part of transgenic plants were obtained after confirmed by RT-PCR amplification.The study showed that the foreign gene TLPD34 has integrated into... |
| |
| Keywords: | Maize Shoot apical point Agrobacterium-mediated TLPD34 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
