宁夏玉米高效再生体系的建立及其影响因素

以4个宁夏玉米品种幼胚为材料,采用组织培养法,研究培养基类型、基因型、外源激素、头孢霉素(Cef)对玉米幼胚培养及再生的影响。结果表明,在愈伤组织诱导过程中,Ⅰ号和Ⅱ号培养基诱导率较高,宁玉12号和宁0816的胚性愈伤组织诱导率相对较高,达到85.3%和82.1%;6-BA对不同基因型愈伤组织分化率的影响不同,添加6-BA对愈伤组织的分化均有促进作用;此外,当Cef质量浓度高于500 mg/L时,愈伤组织分化明显受到抑制。最佳诱导培养基为基本元素N6+水解酪蛋白200 mg/L+脯氨酸690 mg/L+2,4-D 2.0 mg/L+蔗糖30 g/L+肌醇100 mg/L+AgNO310 mg/L,最佳分化培养基为基本培养基N6+6-BA1.0 mg/L+蔗糖50 g/L。

Regeneration System and Influencing Factors of Maize in Ningxia

Using immature embryo of four maize varieties in Ningxia as explants and utilizing the plant tissue culture method,the effect of medium type,genotype,exogenous hormone and cephamycin(Cef) on in vitro culture and regeneration was studied.The results showed that different medium type and genotype showed different ability of embryogenic callus inducing.Ⅰand Ⅱ had more high rate of induction,Ningyu 12 and Ning 0816 had high rate as 85.3% and 82.1% respectively;6-BA were the most important factor for the induction of differentiation of embryogenic callus,6-BA could promote the differentiation of embryogenic callus.Respectively,when 500 mg/L of Cef was added,the differentiation of callus was significant inhibited.The best inducing medium was N6 +Casein hydrolysate 200 mg/L+Pro 690 mg/L+2,4-D 2.0 mg/L + Sugar 30 g/L+Inositol 100 mg/L+AgNO310 mg/L,The best differentiation medium was: N6+6-BA1.0 mg/L+Sugar 50 g/L.