| 山羊子宫内膜腺上皮细胞的分离培养和鉴定 |
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| 引用本文： | 丰艳妮,吴庆侠,吴瑞,楚元奎,靳亚平.山羊子宫内膜腺上皮细胞的分离培养和鉴定[J].西北农业学报,2006,15(6):12-14. |
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| 作者姓名： | 丰艳妮 吴庆侠 吴瑞 楚元奎 靳亚平 |
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| 作者单位： | 农业部家畜生殖内分泌与胚胎工程重点开放实验室,西北农林科技大学动物科技学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 为了探讨山羊子宫内膜腺上皮细胞的分离、生长条件以及纯度鉴定,通过2 g/L的Ⅰ型胶原酶置于水浴消化4 h,200目的滤网过滤后进行低速离心,收集沉淀物,沉降洗涤3~5次,将细胞移至24孔培养板置37℃细胞培养箱中进行培养。12 h后开始贴壁生长,腺团开始向外部扩散,生长2~3 d,细胞扩散并出现明显的铺路石状,4~5 d腺团基本扩散开来并呈一定走向继续生长。经免疫组织化学方法进行细胞染色,细胞表达角蛋白的阳性率达90%以上。
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| 关 键 词： | 子宫内膜 腺上皮细胞 免疫组织化学 鉴定 |
| 文章编号： | 1004-1389（2006）06-0012-03 |
| 收稿时间： | 2006-04-28 |
| 修稿时间： | 2006-06-01 |
Isolation,Culture and Identification of Endometrium Glands in Goats |
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| FENG Yan-ni,WU Qing-xi,WU Rui,CHU Yuan-kui and JIN Ya-ping.Isolation,Culture and Identification of Endometrium Glands in Goats[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(6):12-14. |
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| Authors: | FENG Yan-ni WU Qing-xi WU Rui CHU Yuan-kui and JIN Ya-ping |
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| Institution: | Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | |
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| Keywords: | Endometrium Glands Immunocytochemical Identification |
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