| 添加V_(B_(12))、V_E、V_C对无角道塞特绵羊精液冷冻效果的影响 |
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| 引用本文： | 权富生,黄 欣,马会明,邓志诚,王立华,窦 航.添加V_(B_(12))、V_E、V_C对无角道塞特绵羊精液冷冻效果的影响[J].西北农业学报,2010,19(11):13-17. |
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| 作者姓名： | 权富生 黄 欣 马会明 邓志诚 王立华 窦 航 |
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| 作者单位： | 1. 西北农林科技大学,动物医学院,农业部家畜生殖生理与胚胎工程重点开放实验室,陕西杨凌,712100
2. 杨凌科元克隆股份有限公司,陕西杨凌,712100 |
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| 基金项目： | 杨凌科技重点推广项目“无角道塞特肉绵羊杂交改良及相关配套技术推广”(YLTG2006-2-32) |
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| 摘 要： | 探讨在无角道塞特绵羊冷冻精液稀释液中添加VB12、VE、VC对精子冷冻品质的影响。在无角道塞特绵羊冷冻精液稀释液中添加不同质量浓度的VB12、VE、VC制作细管冻精,解冻后观察精子的成活率和顶体完整率等指标。结果显示,VB12添加0.003 g/L时能够显著提高解冻后的精子活率(P0.05)和精子顶体完整率(P0.05);VE添加2.5 g/L时,能够极显著提高解冻后的精子活率(P0.01)和顶体完整率(P0.01);VC的添加4.4 g/L时能够显著提高解冻后的精子活率(P0.05),但对冷冻后精子的顶体完整率无影响。在稀释液中添加VB12、VE和VC能使解冻后精子品质得到提高。VE添加后能够极显著提高冷冻精子的顶体完整率,应该优先考虑添加。
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| 关 键 词： | 细管冻精 VB12 VE VC 无角道塞特绵羊 |
Effects of Adding VB12,VE and VC in the Frozen Semen Diluent of Poll Dorset Sheep |
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| QUAN Fusheng,HUANG Xin,MA Huiming,DENG Zhicheng,WANG Lihua and DOU Hang.Effects of Adding VB12,VE and VC in the Frozen Semen Diluent of Poll Dorset Sheep[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(11):13-17. |
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| Authors: | QUAN Fusheng HUANG Xin MA Huiming DENG Zhicheng WANG Lihua and DOU Hang |
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| Institution: | QUAN Fusheng1,HUANG Xin1,MA Huiming1,DENG Zhicheng1,WANG Lihua1 and DOU Hang2(1.Key Laboratory of Animal Reproductive Physiology & Embryo Technology of Ministry of Agriculture,College of Veterinary Medicine,Northwest A & F University,Yangling Shaanxi 712100,China,2.Yangling Keyuan Cloning Co.Ltd.Yangling Shaanxi 712100,China) |
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| Abstract: | This study aimed to evaluate the efforts of vitamin B12,vitamin E and vitamin C in the frozen semen diluent of Poll Dorset sheep on the quality of frozen semen.Different concentrations of vitamin B12,vitamin E,vitamin C were added to the frozen semen diluent of Poll Dorset sheep,producing frozen semen straws and observing the indictors such as sperm viability and acrosome integrity of thawed sperm.When the concentration of vitamin B12 was 0.003 g/L,acrosome integrity(P<0.05)and viability of thawed sperm(P<0... |
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| Keywords: | Frozen semen straws VB12 VE VC Poll dorset sheep |
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