| 海南黑山羊鞭虫rDNA-ITS片段的克隆及序列分析 |
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| 引用本文： | 荣 光,周汉林,侯冠彧,王东劲,赵军明.海南黑山羊鞭虫rDNA-ITS片段的克隆及序列分析[J].西北农业学报,2010,19(12):27-30. |
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| 作者姓名： | 荣 光 周汉林 侯冠彧 王东劲 赵军明 |
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| 作者单位： | 1. 中国热带农业科学院,热带作物品种资源研究所,海南儋州,571737
2. 中国热带农业科学院,科技信息研究所,海南儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(PZS056) |
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| 摘 要： | 采用寄生线虫通用引物NC5和NC2,对海南黑山羊鞭虫rDNA的ITS序列进行PCR扩增,将扩增片段纯化后克隆至pEASY-T1载体。用PCR及酶切进行鉴定,对阳性菌落质粒DNA进行测序分析。结果表明,扩增的黑山羊鞭虫ITS片段大小为1 113 bp,包含部分的18S、28S及全部的ITS1(470 bp)、5.8S(158bp)和ITS2(392 bp)序列,ITS1和ITS2与GenBank已登录的Trichuris skrjabini(Baskakov)同源性分别达到90%和99%,与T.leporis(Froelich)同源性均为84%,而与其他科线虫同源性均较低。由ITS1和ITS2序列构建的系统进化树可知,从海南黑山羊盲肠中分离到的鞭虫ITS1和ITS2均与T.skrjabini处于同一分支。研究结果为海南黑山羊盲肠鞭虫种属的确定及进一步分子生物学研究奠定基础。
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| 关 键 词： | 鞭虫 ITS PCR 克隆 序列分析 |
Cloning and Sequence Analysis of the rDNA ITS of Trichuris sp. in Black Goat in Hainan |
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| RONG Guang,ZHOU Hanlin,HOU Guanyu,WANG Dongjin and ZHAO Junming.Cloning and Sequence Analysis of the rDNA ITS of Trichuris sp. in Black Goat in Hainan[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(12):27-30. |
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| Authors: | RONG Guang ZHOU Hanlin HOU Guanyu WANG Dongjin and ZHAO Junming |
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| Institution: | RONG Guang1,ZHOU Hanlin1,HOU Guanyu1,WANG Dongjin1 and ZHAO Junming2(1.Tropical Crops Genetic Resources Institute,CATAS,Danzhou Hainan 571737,China,2.Scientific and Technical Information Institute,China) |
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| Abstract: | The internal transcribed spacer(ITS)of DNA nucleotide fragments of Trichuris sp.isolated from Black Goat in Hainan region were ampliy by PCR of conserved primers NC5 and NC2.The PCR fragments were purified and cloned into pEASY-T1 vector.The inserts were successfully sequenced,and the results revealed ITS inserts were 1 113 bp in length and consisted of partial 18S,28S and complete ITS1(470 bp),5.8S(158 bp) and ITS2(392) rDNA sequences.ITS1 and ITS2 sequences shared 90%,99% homology with T.skrjabini(Baskako... |
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| Keywords: | Trichuris Internal transcribed spacer PCR Cloning Sequence analysis |
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