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| 沙门氏菌常见H抗原特异相的PCR快速检测 | |
| 引用本文： | 刘华伟,马立农,郭蔼光,邱立.沙门氏菌常见H抗原特异相的PCR快速检测[J].西北农业学报,2006,15(2):38-41. |
| 作者姓名： | 刘华伟 马立农 郭蔼光 邱立 |
| 作者单位： | 1. 西北农林科技大学,陕西杨凌,712100;深圳职业技术学院,广东深圳,518055 2. 深圳职业技术学院,广东深圳,518055 3. 西北农林科技大学,陕西杨凌,712100 |
| 摘 要： | 运用PCR技术检测沙门氏菌常见的H抗原特异相,甲型副伤寒、猪霍乱、鼠伤寒等常见危害人和动物的沙门氏菌因H抗原差异而分别获得特异性扩增。此技术具有快速简便、敏感性高和特异性强等优点,值得在对常见危害人和动物的沙门氏菌的快速检测中推广应用。 |
| 关 键 词： | 沙门氏菌 H抗原 快速检测 |
| 文章编号： | 1004-1389（2006）02-00038-04 |
| 收稿时间： | 2005-05-12 |
| 修稿时间： | 2005-12-28 |
Rapid Detection of Salmonella with Familiar Special Phase of H Antigen |
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| LIU Hua-wei,MA Li-nong,GUO Ai-guang and QIU Li.Rapid Detection of Salmonella with Familiar Special Phase of H Antigen[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(2):38-41. | |
| Authors: | LIU Hua-wei MA Li-nong GUO Ai-guang and QIU Li |
| Institution: | Northwest A & F University, Yangling Shaanxi 712100, China;Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China;Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China;Northwest A & F University, Yangling Shaanxi 712100, China;Northwest A & F University, Yangling Shaanxi 712100, China |
| Abstract: | In this article we detected specific primers of special phase of H antigen of Salmonella.S.paratyphi A,S.choleraesuis,S.typhimurium et al were accurately identified by the specific amplification products.The result showed that PCR is a kind of rapid,sensitive and high specificity method to detect Salmonella,which can be well popularized in detection for familiar Salmonella samples. |
| Keywords: | PCR |
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