| 转酿酒酵母HOG1基因拟南芥植株的获得与检测 |
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| 引用本文： | 岳 华,杜向红,吕树作,方桂英,李 康,王 乐,聂小军,宋卫宁.转酿酒酵母HOG1基因拟南芥植株的获得与检测[J].西北农业学报,2010,19(4):86-90. |
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| 作者姓名： | 岳 华 杜向红 吕树作 方桂英 李 康 王 乐 聂小军 宋卫宁 |
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| 作者单位： | 西北农林科技大学,农学院,陕西杨凌,712100;陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 教育部科技创新工程重大项目培育基金，高等学校学科创新引智计划，国家"十一五"、"863"计划 |
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| 摘 要： | HOG1(high osmolarity glycerol,HOG1)是酵母中参与耐高渗透压调控的重要基因。根据已发表的酿酒酵母序列,设计特异引物,扩增到完整的HOG1基因;构建植物表达载体pBI121-HOG1,通过农杆菌介导转化拟南芥,获得转化植株种子。对收获的T0代种子进行卡那霉素抗性筛选,并通过PCR和RT-PCR对抗性苗进行检测。结果表明,构建的载体成功转化拟南芥,并获得了13份转HOG1基因苗。
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| 关 键 词： | HOG1基因 构建植物表达载体 拟南芥转化 |
Transformation of Arabidopsis thaliana with Saccharomyces cerevisiae HOG1 Gene |
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| YUE Hua,DU Xianghong,L Shuzuo,FANG Guiying,LI Kang,WANG Le,NIE Xiaojun,SONG Weining.Transformation of Arabidopsis thaliana with Saccharomyces cerevisiae HOG1 Gene[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(4):86-90. |
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| Authors: | YUE Hua DU Xianghong L Shuzuo FANG Guiying LI Kang WANG Le NIE Xiaojun SONG Weining |
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| Institution: | YUE Hua,DU Xianghong,L(U) Shuzuo,FANG Guiying,LI Kang,WANG Le,NIE Xiaojun,SONG Weining |
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| Abstract: | HOG1 is an important regulatory factor which regulates response to high osmolarity in Yeast.In this paper,the full-length HOG1 gene from the genomic DNA ofSaccharomyces cerevisiae was isolated with PCR technique using primers designed according to the published sequence.To identify potential function of this gene in plant,expression vector pBI121-HOG1 was constructed,and then transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method.T0 transgenic seeds were screened on MS plate cont... |
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| Keywords: | HOG1 gene Construction of plant expression vector Arabidopsis thaliana transformation |
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