新疆天池放线菌的分离及其抑菌活性

为了充分挖掘和利用新疆特色放线菌资源,开发新型微生物源农药,采用选择性分离方法对新疆天池放线菌进行分离,结合离体和活体试验筛选抑菌活性较高的放线菌,并利用形态学和分子生物学手段进行菌株鉴定。结果共分离得到213株放线菌,其中从天池草甸土中得到的放线菌数量最多,占40.38%;离体活性测定结果显示,有5株放线菌对立枯丝核菌和番茄灰霉病菌均具有较好的抑制作用;盆栽试验结果显示,放线菌TC-50对黄瓜立枯病的防治效果最高,为89.98%,并且可提高黄瓜种子的发芽率,促进黄瓜幼苗生长;经鉴定,放线菌TC-50属于链霉菌(Streptomyces sp.)。可见,链霉菌TC-50有望开发为具有新疆特色的微生物源农药。

Isolation and Antifungal Activity of Actinomycetesfrom Tianchi in Xinjiang

In order to excavate elite actinomycete resource from Xinjiang for developing novel microbial pesticides, we isolated actinomycetes from soil of Tianchi, Xinjiang, and tested the antifungal activity in vitro and in vivo. The isolates with strong activity were classified using morphology and molecular biology methods. The results showed that 213 strains of actinomycetes were obtained, and 40.38% of them isolated from meadow soil. Five strains had strong inhibition activities against Rhizoctonia solani and Botrytis cinerea.The strain TC-50 showed a strong efficiency against Rhizoctonia rot of cucumber with the control effect of 89.98%, which also promoted seed germination and seedling growth of cucumber;the strain TC-50, classified as a Streptomyces sp., has a potential to be developed as a microbial pesticide in Xinjiang province.