陕西菜田2种粉虱数量结构及烟粉虱生物型鉴定

旨在研究陕西菜田烟粉虱和白粉虱的数量结构,为粉虱风险评估和防治策略制定提供理论依据。利用定点调查与普查相结合的方法,对陕西关中、陕南和陕北不同生态区、不同季节、不同蔬菜作物以及不同菜田烟粉虱和温室白粉虱的种群分布和组成进行研究,并结合mtDNA-CO1标记基因实现对陕西菜田烟粉虱的生物型鉴定。结果表明,烟粉虱和温室白粉虱的分布和种群组成因地区、季节、作物种植模式不同而异。其中烟粉虱均呈现优势分布,并占到种群数量的67.2%～100%。采用标记基因技术、序列同源性分析和系统发育研究,明确了陕西菜田烟粉虱生物型为Q型烟粉虱。

Ppopulation Composition of Gennadius and Dominant Biotype in Vegetable Field in Shaanxi Province

In order to determine the population composition and distribution of Bemisia tabaci and Trialeurodes vaporariorum Gennadius, and provide the theoretical basis for risk assessment, prevention and control, Bemisia tabaci and Trialeurodes vaporariorum Gennadius were collected from different ecological areas, seasons, vegetables and planting patterns in South, Middle and North of Shaanxi province, to analyze their composition and distribution with mathematical methods. In addition, the marker gene of mtDNA-CO1 was used for the biotypes identification of Bemisia tabaci Gennadius. The results indicated that the population proportion and distribution of Bemisia tabaci and Trialeurodes vaporariorum Gennadius were influenced by the different host vegetables and climatic factors. For Bemisia tabaci Gennadius, they showed the dominant distribution (67.2%-100%) in whole field. Interestingly, this kind of Bemisia tabaci Gennadius were ifentified as biotype Q followed the gene amplification, sequences alignment and phylogenetic analysis.