枸杞种间杂交F_1群体的SSR鉴定及遗传分析

以黄果枸杞(Lyciumbararumvar.auranticarpum,用P_1表示)为父本,北方枸杞(L.chinensevar.potaninii,用P_2表示)为母本,杂交获得F_1群体91个单株。从66对SSR引物中筛选出具有双亲互补型杂合位点6对引物,对91个F_1单株进行杂种鉴定和遗传变异分析。结果表明:6对引物在91个单株进行扩增检测结果中有83个单株在6个SSR位点上表现为双亲互补型杂合位点,可被认定为真杂种,杂种率为91.2%,另外8个单株出现异常SSR基因型,需要结合细胞学进一步验证。UPGMA聚类分析结果显示:在遗传距离为0.712处,83个F_1单株被划分为2大类,第Ⅰ大类包括38个F_1单株,占45.8%,第二大类包括45个F_1单株,占54.2%。杂交后代遗传变异大,遗传多样性丰富。

Hybrid Identification and Genetic Analysis in Wolfberry F1 Population Using SSR Markers

A total of 91 plants of F₁ population were obtained by crossing L.bararum var.auranticarpum as a male parent(expressed in P₁) with L.chinense var.potaninii as a female parent(expressed in P₂).Six pairs of SSR primers with parental complementary heterozygous loci were screened out from 66 pairs of SSR primers for hybridization identification and genetic variation analysis of those 91 plants.Six pairs of primers were used for amplification experiments.The results indicated that 83 of 91 plants showed parental complementary heterozygous loci on these 6 SSR loci with the heterozygous rate of 91.2%,which could be identified as true hybrids.The other 8 plants showed abnormal SSR genotypes,which needed further verification with cytology.Further UPGMA cluster analysis revealed that the 83 plants of F₁ population were divided into two groups at genetic distance of 0.712.The first group included 38 plants(45.8%) and the second group included 45 plants(54.2%).Hybrid progenies have great genetic variation and rich genetic diversity.