富含多糖葡萄风信子花瓣总RNA提取方法研究

在普通CTAB法与SDS法提取RNA的基础上,从去除多糖和DNA污染两个方面进行优化,筛选出适于富含多糖类葡萄风信子花瓣RNA提取的方法,经检测OD260/280值在1.8～2.0之间,电泳28S条带和18SrRNA条带都很清亮,比值约1.5.对改进的CTAB法提取的总RNA进行RT-PCR能扩增出所需目的条带.有效去除多糖类物质污染对于富含多糖类花瓣组织RNA提取很重要.

Research of Total RNA Extraction Methods from Polysaccharideriched Petals of Common Grape Hyacinth

Extracting high-quality RNA from common grape hyacinth flowers using common methods is difficult because of high levels of polysaccharide in flowers.Total RNA was isolated from common grape hyacinth with modified CTAB method and SDS method.UV-Spectroscopic analysis showed that A(260)/A(280)= 1.8~2.0,28S,18SrRNA were all clear in electrophoresis picture.Target gene was amplified from isolated total RNA by RT-PCR which testified high quality could be isolated from petals with modified methods.In conclusion,it is pretty important to reduce polysaccharide for obtaining high quality total RNA from polysaccharide-riched flower petals.