复方蜂胶纳米乳的制备及其品质评价

制备复方蜂胶纳米乳并进行品质评价。利用伪三元相图法优选处方,以RH-40、无水乙醇、乙酸乙酯、蜂胶和黄芪多糖为原料制备复方蜂胶纳米乳,并考察其结构类型、形态粒径、黏度、稳定性。结果表明:复方蜂胶纳米乳透射电镜下呈球形,平均粒径为12.70 nm,粒径分布均匀,黏度2 s(每垂直流出0.4 mL所需时间)。影响因素试验显示其品质稳定。复方蜂胶纳米乳制备工艺简单、稳定性好、黏度低,有望成为疫苗佐剂的新型制剂。

Preparation and Quality Evaluation of Compound Propolis Nanoemulsion

The Compound Propolis Nanoemulsion(CPN) was prepared and its quality was evaluated. Optimized formula of CPN was produced with the method of the pseudoternary phase diagrams. RH 40, absolute alcohol, ethyl acetate, propolis, astragalus polysaccharides and distilled water were selected to prepare CPN. Its structural type, appearance, particle size, viscosity, and physical stability were tested respectively. CPN presented as spherical drops with uniform size distribution and mean diameter of 12.70 nm in the observation of the transmission electron microscope(TEM) and Nicomp388/Zeta PALS. The viscosity of CPN was 2 s · 0.4 mL^-1 and good physical stability was proved by influencing factors experiment. The Compound Propolis nanoemulsion is a promising vaccine adjuvant for its simple preparation procedure, good stability, and low viscosity.