利用SSR标记技术分析中国啤酒大麦品种的遗传多样性

利用SSR标记技术对中国38个啤酒大麦品种的遗传背景进行了遗传多样性分析.结果表明,所用28对SSR引物（在大麦7对染色体长、短臂位置各选用了2对）中,有26对SSR引物有多态性,共检测出187个位点,其中172个等位位点有多态性,占91.9%;每个引物可扩增出1～22个位点,平均每个引物产生6.6个等位位点.26对SSR引物的多态性信息含量PIC最高为0.995,最低为0.375,平均PIC为0.713.聚类结果表明,这些品种的遗传距离（GD）聚类范围较小,分布范围在0.01124～0.74510,在遗传距离GD值0.6水平上,这些品种聚成了2大类,下分4个亚类.SSR标记揭示出这38个啤酒大麦品种遗传变异较小,遗传基础比较狭窄.

Analysis of Genetic Diversity on Beer Barley Varieties in China by SSR

The genetic relationships among 38 beer barley from China were investigated by SSR. The results showed that choosing 2 pairs separately in the long as well as short arms on the 7 pairs of chromosome in barley, in all, we got 28 pairs of SSR primer. 26 pairs of SSR primer were detected to have polymorphism and 187 loci were measured totally. Therein, 172 loci have polymorphism which occupies 91.9%. Each primer can augment 1-22 loci with 6.6 loci in the average. The polymorphism information content among the 26 pairs got the highest PIC 0. 995 and the lowest 0. 375, with the average 0. 713 among the 26 pairs. Cluster analysis showed that 38 beer barley could be classified into 2 groups at the level of GD 0.6, which were respectively classified into 4 subgroups again. The GD varied from 0. 01124 to 0. 74510. Most beer barley had showed certain regular distribution in every subgroup. The results indicated that the genetic basis of them was rather narrow.