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| 子房注射法获得转基因抗虫棉植株研究 | |
| 引用本文： | 张震林,倪万潮,郭三堆,束春娥,崔洪志,张保龙,徐英俊.子房注射法获得转基因抗虫棉植株研究[J].西北农业学报,2000,9(2):1-4. |
| 作者姓名： | 张震林 倪万潮 郭三堆 束春娥 崔洪志 张保龙 徐英俊 |
| 作者单位： | 1. 江苏省农业科学院经济作物研究所,南京,210014 2. 中国农业科学院生物技术研究中心,北京,100081 3. 江苏省农业科学院植物保护研究所,南京,210014 |
| 摘 要： | 采用子房注射法将构建在植物表达载体ｐＢＩ１２１．１上的Ｂｔ杀虫基因导入棉花品种石远３２１、中植３７２、８２２和辽棉９００１中，获得８株转基因植株。经温室抗虫性鉴定，８株转基因植株对棉铃虫都表现出较好的抗性。同时对这８棵株叶片进行的ＰＣＲ检测结果证明Ｂｔ基因已经整合进植物基因组中。 |
| 关 键 词： | 棉花 Bt杀虫基因 棉铃虫 抗虫 子房注射法 |
| 收稿时间： | 1999/8/21 0:00:00 |
| 修稿时间： | 1999－08－21 |
Insect resistant Transgenic Cotton Plants Transformed with Pollen Tube Pathway |
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| ZHANG Zhen-lin,NI Wan-chao,GUO San-dui,SHU Chun-e,CUI Hong-zhi,ZHANG Bao-long and XU Ying-jun.Insect resistant Transgenic Cotton Plants Transformed with Pollen Tube Pathway[J].Acta Agriculturae Boreali-occidentalis Sinica,2000,9(2):1-4. | |
| Authors: | ZHANG Zhen-lin NI Wan-chao GUO San-dui SHU Chun-e CUI Hong-zhi ZHANG Bao-long and XU Ying-jun |
| Institution: | Institute of Industrial Crops, Jisngsu Academy of Agricultural Sciences, Nanjing 210014;Institute of Industrial Crops, Jisngsu Academy of Agricultural Sciences, Nanjing 210014;Research Center of Biotechnology Chinese Academy of Agricultural Sciences, Beijing 100081;Institute of Plant Protection, Jiangsu Academy of A gricultural Sciences, Nanjing 210014;Research Center of Biotechnology Chinese Academy of Agricultural Sciences, Beijing 100081;Institute of Industrial Crops, Jisngsu Academy of Agricultural Sciences, Nanjing 210014;Institute of Industrial Crops, Jisngsu Academy of Agricultural Sciences, Nanjing 210014 |
| Abstract: | By in jecting a synthetic Btcrystal-proteingene,GFMC ry1A,cloned in a plant expression vector,pBI121.1,into ovaries of cotton varieties Shiyuan321,Zhongzhi372,822 and Liaomian9001,8Bt-transgenic plants were obtained.Aboassay was performed in a greenhouse.The plants showed satisfactory resistance to larvae of bollworm.The positive result of PCR proved that the Bt gene had been in tegrated into cotton genome.These results indicated that such direct transformation in situ as an ovary-injection mathod was efficient. |
| Keywords: | G hirsutum L Bttran sgene Helicoverpa armigera Hubner |
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