发情期山羊腺垂体中IFN α和IFN γ 的免疫组化研究

为观察干扰素(IFN)的分布与山羊腺垂体内分泌细胞在发情免疫调节中的形态学依据。运用免疫组织化学SP法分别对发情前期、发情期、发情后期和间情期山羊腺垂体内IFN-α和IFN-γ阳性细胞的分布和变化特点进行研究。结果显示,2种干扰素阳性物质主要分布于腺垂体嗜酸性细胞、嗜碱性细胞和嫌色细胞的细胞质,远侧部和结节部较多;不同发情期IFN-α和IFN-γ的分布存在明显差异,IFN-α相对表达量均高于IFN-γ,但两者在发情周期中的变化趋势是一致的;发情前期和间情期相对表达量较高,后期最低,发情期呈中等强度表达。表明IFN-α和IFN-γ各自在发情的不同时期发挥作用或相互协同调节垂体激素的合成与分泌,进而参与动物的生殖调节。

Immunohistochemistry Research of Interferon-α and Interferon-γ in Adenohypophysis of Goat under Different Oestrum

To study the distribution of interferon-α and interferon-γ in adenohypophysis of goat under different oestrum and to determine morphologic change of oestrum immunoregulation,immunochemistry SP method was used to test distribution characteristics of interferon-α and interferon-γ.The result showed that IFN-α and IFN-γ cells are located in cytoplasm of eosinophil cells,basicyte and chromophobe cells.Moreover,the positive cells mostly distribute in distal portion and tuberal part.IFN-α and IFN-γ exist significant discrepancy in different oestrum.The relative expression of IFN-α is greater than that of IFN-γ,but the variation trend is accordant,i.e.,high expression in oestrous prophase and dioestrum,and lower in oestrous and oestrous anaphase.The results indicates that IFN-α and IFN-γ involve in animal reproduction control through their functions individually or together at different oestrum stage to regulate synthesis and secretion of hypophyseal hormone.